Abstract
We hypothesised that the measurement of circulating collagen-degrading matrix metalloproteinases (MMPs) would be predictive of postsurgical outcome after fasciectomy for Dupuytren disease (DD), measured through total extension deficit (TED). Assay of MMP-1, MMP-13 and MMP-14 in the plasma of DD patients showed poor correlation with change in TED after surgery. However, MMP-14 levels correlated with preoperative TED. In order to extend this study, we also measured the transcriptome in tissue samples from DD patients and showed that expression of specific genes correlated with preoperative and postoperative TED. These methodologies demonstrate the use of the approach in uncovering biomarkers which both illuminate the mechanism of the disease and have clinical utility.
| Original language | English |
|---|---|
| Title of host publication | Dupuytren Disease and Related Diseases - The Cutting Edge |
| Editors | Paul M. N. Werker, Joseph Dias, Charles Eaton, Bert Reichert, Wolfgang Wach |
| Publisher | Springer |
| Pages | 55-61 |
| Number of pages | 7 |
| ISBN (Electronic) | 978-3-319-32199-8 |
| ISBN (Print) | 978-3-319-32197-4 |
| DOIs | |
| Publication status | Published - 7 Sep 2016 |
Keywords
- Gene expression
- qRT-PCR
- ELISA
- Microarray
- Biomarker
- Recurrence
Profiles
-
Ian Clark
- School of Biological Sciences - Professor of Musculoskeletal Biology
- Vice-Chancellor's Office - HealthUEA Academic Chair
- Musculoskeletal Medicine - Member
- Nutrition and Preventive Medicine - Member
- HealthUEA - Academic Chair
Person: Research Group Member, Academic, Teaching & Research