Biophysical and electrochemical studies of protein-nucleic acid interactions

Richard Bowater, Andrew Cobb, Hana Pivonkova, Ludek Havran, Miroslav Fojta

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This review is devoted to biophysical and electrochemical methods used for studying protein-nucleic acid (NA) interactions. The importance of NA structure and protein-NA recognition for essential cellular processes, such as replication or transcription, is discussed to provide background for description of a range of biophysical chemistry methods that are applied to study a wide scope of protein-DNA and protein-RNA complexes. These techniques employ different detection principles with specific advantages and limitations and are often combined as mutually complementary approaches to provide a complete description of the interactions. Electrochemical methods have proven to be of great utility in such studies because they provide sensitive measurements and can be combined with other approaches that facilitate the protein-NA interactions. Recent applications of electrochemical methods in studies of protein-NA interactions are discussed in detail.
Original languageEnglish
Pages (from-to)723-739
Number of pages17
JournalMonatshefte für Chemie - Chemical Monthly
Issue number5
Early online date3 Feb 2015
Publication statusPublished - May 2015


  • Bioorganic chemistry
  • Biosensors
  • Electrochemistry
  • Electron transfer
  • Electrophoresis
  • Nucleic acids
  • Proteins
  • Voltammetry

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