TY - JOUR
T1 - c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE (Journal of Biological Chemistry (2010) 285, (22882-22889))
AU - Goddard, Alan D.
AU - Stevens, Julie M.
AU - Rao, Feng
AU - Mavridou, Despoina A.I.
AU - Chan, Weelee
AU - Richardson, David J.
AU - Allen, James W.A.
AU - Ferguson, Stuart J.
PY - 2011/8/19
Y1 - 2011/8/19
N2 - The Ccm cytochrome c maturation System I catalyzes covalent attachment of heme to apocytochromes c in many bacterial species and some mitochondria. A covalent, but transient, bond between heme and a conserved histidine in CcmE along with an interaction between CcmH and the apocytochrome have been previously indicated as core aspects of the Ccm system. Here, we show that in the Ccm system from Desulfovibrio desulfuricans, no CcmH is required, and the holo-CcmE covalent bond occurs via a cysteine residue. These observations call for reconsideration of the accepted models of System I-mediated c-type cytochrome biogenesis.
AB - The Ccm cytochrome c maturation System I catalyzes covalent attachment of heme to apocytochromes c in many bacterial species and some mitochondria. A covalent, but transient, bond between heme and a conserved histidine in CcmE along with an interaction between CcmH and the apocytochrome have been previously indicated as core aspects of the Ccm system. Here, we show that in the Ccm system from Desulfovibrio desulfuricans, no CcmH is required, and the holo-CcmE covalent bond occurs via a cysteine residue. These observations call for reconsideration of the accepted models of System I-mediated c-type cytochrome biogenesis.
UR - http://www.scopus.com/inward/record.url?scp=80051700975&partnerID=8YFLogxK
U2 - 10.1074/jbc.A110.133421
DO - 10.1074/jbc.A110.133421
M3 - Comment/debate
AN - SCOPUS:80051700975
VL - 286
SP - 29442
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 33
ER -