Calcipotriol inhibits rectal epithelial cell proliferation in ulcerative proctocolitis

M G Thomas, K P Nugent, A Forbes, R C Williamson

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Vitamin D3 reduces human rectal crypt cell production rate (CCPR) and may thereby protect against colorectal cancer. Cell turnover is increased in ulcerative proctocolitis, which might therefore respond to vitamin D3 metabolites. This study investigated the effect of calcipotriol, a synthetic vitamin D3 analogue that avoids hypercalcaemia, on human rectal CCPR in ulcerative proctocolitis. Paired rectal biopsy specimens from seven patients with severe disease were established in organ culture with or without calcipotriol (1 x 10(-6) M). After 15 hours, vincristine (0.6 microgram/ml) was added to induce metaphase arrest, and CCPR was determined by linear regression analysis of accumulated metaphases. Compared with values in 17 controls with incidental anal conditions, median rectal CCPR was 28% higher in ulcerative proctocolitis: 5.90 (5.00-9.50) v 4.80 (2.85-7.07) cells/crypt/hour (p
Original languageEnglish
Pages (from-to)1718-1720
Number of pages3
JournalGut
Volume35
Issue number12
DOIs
Publication statusPublished - Dec 1994

Keywords

  • Adult
  • Aged
  • Aged, 80 and over
  • Calcitriol
  • Cell Division
  • Colitis, Ulcerative
  • Colorectal Neoplasms
  • Depression, Chemical
  • Epithelium
  • Female
  • Humans
  • Male
  • Middle Aged
  • Organ Culture Techniques

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