Calcium-supplemented daptomycin Etest strips for susceptibility testing on Iso-Sensitest agar

Alan P Johnson, Shazad Mushtaq, Marina Warner, David M Livermore

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Iso-Sensitest agar (ISA), which is recommended by the BSAC for routine susceptibility testing of staphylococci and enterococci, contains insufficient calcium for testing daptomycin. Isotonic agar supplemented with 50 mg/L calcium has been advocated, but is not routinely available in many laboratories. We evaluated a daptomycin Etest that incorporates a constant level of calcium throughout the daptomycin gradient, designed to give an appropriate concentration around the strip during testing, as an alternative for susceptibility testing on ISA.
Original languageEnglish
Pages (from-to)860-2
Number of pages3
JournalJournal of Antimicrobial Chemotherapy
Issue number5
Publication statusPublished - May 2004


  • Anti-Bacterial Agents
  • Calcium
  • Culture Media
  • Daptomycin
  • Enterococcus
  • Gram-Positive Bacterial Infections
  • Great Britain
  • Humans
  • Methicillin Resistance
  • Microbial Sensitivity Tests
  • Reagent Strips
  • Staphylococcus aureus

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