Calcium-supplemented daptomycin Etest strips for susceptibility testing on Iso-Sensitest agar

Alan P Johnson; Shazad Mushtaq; Marina Warner; David M Livermore

doi:10.1093/jac/dkh198

Calcium-supplemented daptomycin Etest strips for susceptibility testing on Iso-Sensitest agar

Alan P Johnson
, Shazad Mushtaq
, Marina Warner
, David M Livermore

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Iso-Sensitest agar (ISA), which is recommended by the BSAC for routine susceptibility testing of staphylococci and enterococci, contains insufficient calcium for testing daptomycin. Isotonic agar supplemented with 50 mg/L calcium has been advocated, but is not routinely available in many laboratories. We evaluated a daptomycin Etest that incorporates a constant level of calcium throughout the daptomycin gradient, designed to give an appropriate concentration around the strip during testing, as an alternative for susceptibility testing on ISA.

Original language	English
Pages (from-to)	860-2
Number of pages	3
Journal	Journal of Antimicrobial Chemotherapy
Volume	53
Issue number	5
DOIs	https://doi.org/10.1093/jac/dkh198
Publication status	Published - May 2004

Keywords

Anti-Bacterial Agents
Calcium
Culture Media
Daptomycin
Enterococcus
Gram-Positive Bacterial Infections
Great Britain
Humans
Methicillin Resistance
Microbial Sensitivity Tests
Reagent Strips
Staphylococcus aureus

Access to Document

10.1093/jac/dkh198

Cite this

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title = "Calcium-supplemented daptomycin Etest strips for susceptibility testing on Iso-Sensitest agar",

abstract = "Iso-Sensitest agar (ISA), which is recommended by the BSAC for routine susceptibility testing of staphylococci and enterococci, contains insufficient calcium for testing daptomycin. Isotonic agar supplemented with 50 mg/L calcium has been advocated, but is not routinely available in many laboratories. We evaluated a daptomycin Etest that incorporates a constant level of calcium throughout the daptomycin gradient, designed to give an appropriate concentration around the strip during testing, as an alternative for susceptibility testing on ISA.",

keywords = "Anti-Bacterial Agents, Calcium, Culture Media, Daptomycin, Enterococcus, Gram-Positive Bacterial Infections, Great Britain, Humans, Methicillin Resistance, Microbial Sensitivity Tests, Reagent Strips, Staphylococcus aureus",

author = "Johnson, \{Alan P\} and Shazad Mushtaq and Marina Warner and Livermore, \{David M\}",

year = "2004",

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T1 - Calcium-supplemented daptomycin Etest strips for susceptibility testing on Iso-Sensitest agar

AU - Johnson, Alan P

AU - Mushtaq, Shazad

AU - Warner, Marina

AU - Livermore, David M

PY - 2004/5

Y1 - 2004/5

N2 - Iso-Sensitest agar (ISA), which is recommended by the BSAC for routine susceptibility testing of staphylococci and enterococci, contains insufficient calcium for testing daptomycin. Isotonic agar supplemented with 50 mg/L calcium has been advocated, but is not routinely available in many laboratories. We evaluated a daptomycin Etest that incorporates a constant level of calcium throughout the daptomycin gradient, designed to give an appropriate concentration around the strip during testing, as an alternative for susceptibility testing on ISA.

AB - Iso-Sensitest agar (ISA), which is recommended by the BSAC for routine susceptibility testing of staphylococci and enterococci, contains insufficient calcium for testing daptomycin. Isotonic agar supplemented with 50 mg/L calcium has been advocated, but is not routinely available in many laboratories. We evaluated a daptomycin Etest that incorporates a constant level of calcium throughout the daptomycin gradient, designed to give an appropriate concentration around the strip during testing, as an alternative for susceptibility testing on ISA.

KW - Anti-Bacterial Agents

KW - Calcium

KW - Culture Media

KW - Daptomycin

KW - Enterococcus

KW - Gram-Positive Bacterial Infections

KW - Great Britain

KW - Humans

KW - Methicillin Resistance

KW - Microbial Sensitivity Tests

KW - Reagent Strips

KW - Staphylococcus aureus

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DO - 10.1093/jac/dkh198

M3 - Article

C2 - 15056637

SN - 0305-7453

VL - 53

SP - 860

EP - 862

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

IS - 5

ER -