Candidate tumor suppressor LUCA-15/RBM5/H37 modulates expression of apoptosis and cell cycle genes

Mirna Mourtada-Maarabouni, Jennifer Keen, Jeremy Clark, Colin S Cooper, Gwyn T Williams

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

RBM5 (RNA-binding motif protein 5/LUCA-15/H37) is encoded at the lung cancer tumor suppressor locus 3p21.3 and itself has several important characteristics of a tumor suppressor, including both potentiation of apoptosis and inhibition of the cell cycle. Here, we report the effects of both upregulation and downregulation of LUCA-15/RBM5 on gene expression monitored using cDNA microarrays. Many of the genes modulated by LUCA-15/RBM5 are involved in the control of apoptosis, the cell cycle, or both. These effects were confirmed for the most significant genes using real-time RT-PCR and/or Western blotting. In particular, LUCA-15/RBM5 increased the expression of Stat5b and BMP5 and decreased the expression of AIB1 (Amplified In Breast Cancer 1), proto-oncogene Pim-1, caspase antagonist BIRC3 (cIAP-2, MIHC), and CDK2 (cyclin-dependent kinase 2). These effects on multiple genes controlling both apoptosis and proliferation are in line with the functional effects of LUCA-15/RBM5 and indicate that it plays a central role in regulating cell fate consistent with its tumor suppressor activity.
Original languageEnglish
Pages (from-to)1745-52
Number of pages8
JournalExperimental Cell Research
Volume312
Issue number10
DOIs
Publication statusPublished - 10 Jun 2006

Keywords

  • Animals
  • Apoptosis
  • Cell Cycle
  • Cell Cycle Proteins
  • Cell Line, Tumor
  • DNA-Binding Proteins
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Genes, Tumor Suppressor
  • Genes, cdc
  • Humans
  • Molecular Sequence Data
  • Oligonucleotide Array Sequence Analysis
  • RNA-Binding Proteins
  • Tumor Suppressor Proteins

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