Carotenoid-to-(bacterio)chlorophyll energy transfer in LH2 antenna complexes from Rba. sphaeroides reconstituted with non-native (bacterio)chlorophylls

Dariusz M. Niedzwiedzki, David J. K. Swainsbury, C. Neil Hunter

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Six variants of the LH2 antenna complex from Rba. sphaeroides, comprising the native B800-B850, B800-free LH2 (B850) and four LH2s with various (bacterio)chlorophylls reconstituted into the B800 site, have been investigated with static and time-resolved optical spectroscopies at room temperature and at 77 K. The study particularly focused on how reconstitution of a non-native (bacterio)chlorophylls affects excitation energy transfer between the naturally bound carotenoid spheroidene and artificially substituted pigments in the B800 site. Results demonstrate there is no apparent trend in the overall energy transfer rate from spheroidene to B850 bacteriochlorophyll a; however, a trend in energy transfer rate from the spheroidene S1 state to Qy of the B800 (bacterio)chlorophylls is noticeable. These outcomes were applied to test the validity of previously proposed energy values of the spheroidene S1 state, supporting a value in the vicinity of 13,400 cm−1 (746 nm).

Original languageEnglish
Pages (from-to)155-169
Number of pages15
JournalPhotosynthesis Research
Volume144
Issue number2
Early online date26 Jul 2019
DOIs
Publication statusPublished - 1 May 2020

Keywords

  • Carotenoids
  • Light harvesting
  • Light harvesting complex 2
  • Purple bacteria
  • Spheroidene
  • Transient absorption

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