Cautionary notes on the use of C-terminal BAK1 fusion proteins for functional studies

Vardis Ntoukakis, Benjamin Schwessinger, Cécile Segonzac, Cyril Zipfel

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Abstract

Detailed phenotypic characterization reveals that several BAK1 fusion proteins with C-terminal tags strongly impair complementation of bak1 null mutants with respect to responsiveness to the bacterial pathogen-associated molecular patterns flagellin and EF-Tu. This raises concerns about the widespread use of such protein variants of this important regulatory Leu-rich repeat receptor-like kinase (RLK) for functional analyses of RLK-based signaling.
Original languageEnglish
Pages (from-to)3871-3878
Number of pages8
JournalThe Plant Cell
Volume23
Issue number11
DOIs
Publication statusPublished - Nov 2011

Keywords

  • Arabidopsis
  • Arabidopsis Proteins
  • Brassinosteroids
  • Flagellin
  • Mutation
  • Peptide Elongation Factor Tu
  • Plants, Genetically Modified
  • Protein Kinases
  • Protein-Serine-Threonine Kinases
  • Recombinant Fusion Proteins
  • Signal Transduction

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