Cautionary notes on the use of C-terminal BAK1 fusion proteins for functional studies

Vardis Ntoukakis; Benjamin Schwessinger; Cécile Segonzac; Cyril Zipfel

doi:10.1105/tpc.111.090779

Cautionary notes on the use of C-terminal BAK1 fusion proteins for functional studies

Vardis Ntoukakis, Benjamin Schwessinger, Cécile Segonzac, Cyril Zipfel

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

Detailed phenotypic characterization reveals that several BAK1 fusion proteins with C-terminal tags strongly impair complementation of bak1 null mutants with respect to responsiveness to the bacterial pathogen-associated molecular patterns flagellin and EF-Tu. This raises concerns about the widespread use of such protein variants of this important regulatory Leu-rich repeat receptor-like kinase (RLK) for functional analyses of RLK-based signaling.

Original language	English
Pages (from-to)	3871-3878
Number of pages	8
Journal	The Plant Cell
Volume	23
Issue number	11
DOIs	https://doi.org/10.1105/tpc.111.090779
Publication status	Published - Nov 2011

Keywords

Arabidopsis
Arabidopsis Proteins
Brassinosteroids
Flagellin
Mutation
Peptide Elongation Factor Tu
Plants, Genetically Modified
Protein Kinases
Protein-Serine-Threonine Kinases
Recombinant Fusion Proteins
Signal Transduction

Access to Document

10.1105/tpc.111.090779

Cite this

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abstract = "Detailed phenotypic characterization reveals that several BAK1 fusion proteins with C-terminal tags strongly impair complementation of bak1 null mutants with respect to responsiveness to the bacterial pathogen-associated molecular patterns flagellin and EF-Tu. This raises concerns about the widespread use of such protein variants of this important regulatory Leu-rich repeat receptor-like kinase (RLK) for functional analyses of RLK-based signaling.",

keywords = "Arabidopsis, Arabidopsis Proteins, Brassinosteroids, Flagellin, Mutation, Peptide Elongation Factor Tu, Plants, Genetically Modified, Protein Kinases, Protein-Serine-Threonine Kinases, Recombinant Fusion Proteins, Signal Transduction",

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KW - Arabidopsis

KW - Arabidopsis Proteins

KW - Brassinosteroids

KW - Flagellin

KW - Mutation

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KW - Plants, Genetically Modified

KW - Protein Kinases

KW - Protein-Serine-Threonine Kinases

KW - Recombinant Fusion Proteins

KW - Signal Transduction

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