CCL3 induced migration occurs independently of intracellular calcium release

C. Moyano Cardaba; R. O. Jacques; J. E. Barrett; K. M. Hassell; A. Kavanagh; F. C. Remington; T. Tse; A. Mueller

doi:10.1016/j.bbrc.2011.12.081

CCL3 induced migration occurs independently of intracellular calcium release

C. Moyano Cardaba, R. O. Jacques, J. E. Barrett, K. M. Hassell, A. Kavanagh, F. C. Remington, T. Tse, A. Mueller

Molecular and Tissue Pharmacology

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The molecular signalling pathway of cell migration and whether it can occur independently of the release of intracellular calcium is still not completely understood. Therefore we investigated here the molecular mechanisms of CCL3 induced cell migration and the importance of intracellular calcium for chemotaxis in more detail. We show that CCL3 induced cell migration is dependent on activation of PLC. Several PKC inhibitors block the release of intracellular calcium independently of CCL3 activation and do not affect cell migration. This confirms that the release of intracellular calcium is not necessary for chemotaxis towards CCL3 and that PKC inhibitors should be used with caution in calcium release assays.

Original language	English
Pages (from-to)	17-21
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	418
Issue number	1
Early online date	12 Dec 2011
DOIs	https://doi.org/10.1016/j.bbrc.2011.12.081
Publication status	Published - 3 Feb 2012

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10.1016/j.bbrc.2011.12.081

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title = "CCL3 induced migration occurs independently of intracellular calcium release",

abstract = "The molecular signalling pathway of cell migration and whether it can occur independently of the release of intracellular calcium is still not completely understood. Therefore we investigated here the molecular mechanisms of CCL3 induced cell migration and the importance of intracellular calcium for chemotaxis in more detail. We show that CCL3 induced cell migration is dependent on activation of PLC. Several PKC inhibitors block the release of intracellular calcium independently of CCL3 activation and do not affect cell migration. This confirms that the release of intracellular calcium is not necessary for chemotaxis towards CCL3 and that PKC inhibitors should be used with caution in calcium release assays.",

author = "{Moyano Cardaba}, C. and Jacques, {R. O.} and Barrett, {J. E.} and Hassell, {K. M.} and A. Kavanagh and Remington, {F. C.} and T. Tse and A. Mueller",

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T1 - CCL3 induced migration occurs independently of intracellular calcium release

AU - Moyano Cardaba, C.

AU - Jacques, R. O.

AU - Barrett, J. E.

AU - Hassell, K. M.

AU - Kavanagh, A.

AU - Remington, F. C.

AU - Tse, T.

AU - Mueller, A.

PY - 2012/2/3

Y1 - 2012/2/3

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AB - The molecular signalling pathway of cell migration and whether it can occur independently of the release of intracellular calcium is still not completely understood. Therefore we investigated here the molecular mechanisms of CCL3 induced cell migration and the importance of intracellular calcium for chemotaxis in more detail. We show that CCL3 induced cell migration is dependent on activation of PLC. Several PKC inhibitors block the release of intracellular calcium independently of CCL3 activation and do not affect cell migration. This confirms that the release of intracellular calcium is not necessary for chemotaxis towards CCL3 and that PKC inhibitors should be used with caution in calcium release assays.

U2 - 10.1016/j.bbrc.2011.12.081

DO - 10.1016/j.bbrc.2011.12.081

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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