Cell interactions, signals and transcriptional hierarchy governing placode progenitor induction.

Mark Hintze, Ravindra Singh Prajapati, Monica Tambalo, Nicolas Christophorou, Maryam Anwar, Timothy Grocott, Andrea Streit

Research output: Contribution to journalArticle

18 Citations (Scopus)
10 Downloads (Pure)

Abstract

In vertebrates, cranial placodes contribute to all sense organs and sensory ganglia and arsise from a common pool of Six1/Eya2+ progenitors. Here we dissect the events that specify ectodermal cells as placode progenitors using newly identified genes upstream of the Six/Eya complex. We show that two different tissues, the lateral head mesoderm and the prechordal mesendoderm, gradually induce placode progenitors: cells pass through successive transcriptional states, each identified by distinct factors and controlled by different signals. Both tissues initiate a common transcriptional state, but over time impart regional character and the acquisition of anterior identity depends on Shh signalling. Using a network inference approach we predict the regulatory relationships among newly identified transcription factors and verify predicted links using knock-down experiments. Based on this analysis we propose a new model for placode progenitor induction, in which the initial induction of a generic transcriptional state precedes regional divergence.
Original languageEnglish
Pages (from-to)2810-2823
JournalDevelopment
Volume144
Issue number15
Early online date6 Jul 2017
DOIs
Publication statusPublished - 1 Aug 2017

Keywords

  • chick embryo
  • cell fate
  • sense organs
  • sensory ganglia
  • transcriptional networks
  • signalling

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