Chaperone-like protein DAY plays critical roles in photomorphogenesis

Ho-Seok Lee, Ilyeong Choi, Young Jeon, Hee-Kyung Ahn, Huikyong Cho, Jiwoo Kim, Jae-Hee Kim, Jung-Min Lee, Sunghee Lee, Julian Bünting, Dong Hye Seo, Tak Lee, Du-Hwa Lee, Insuk Lee, Man-Ho Oh, Tae-Wuk Kim, Youssef Belkhadir, Hyun-Sook Pai

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Abstract

Photomorphogenesis, light-mediated development, is an essential feature of all terrestrial plants. While chloroplast development and brassinosteroid (BR) signaling are known players in photomorphogenesis, proteins that regulate both pathways have yet to be identified. Here we report that DE-ETIOLATION IN THE DARK AND YELLOWING IN THE LIGHT (DAY), a membrane protein containing DnaJ-like domain, plays a dual-role in photomorphogenesis by stabilizing the BR receptor, BRI1, as well as a key enzyme in chlorophyll biosynthesis, POR. DAY localizes to both the endomembrane and chloroplasts via its first transmembrane domain and chloroplast transit peptide, respectively, and interacts with BRI1 and POR in their respective subcellular compartments. Using genetic analysis, we show that DAY acts independently on BR signaling and chlorophyll biogenesis. Collectively, this work uncovers DAY as a factor that simultaneously regulates BR signaling and chloroplast development, revealing a key regulator of photomorphogenesis that acts across cell compartments.
Original languageEnglish
Article number4194
JournalNature Communications
Volume12
Issue number1
DOIs
Publication statusPublished - 7 Jul 2021

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