Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2

Claire Kidgell; Derek Pickard; John Wain; Keith James; L. T. Diem Nga; To Song Diep; Myron M. Levine; Peadar O'Gaora; Michael B. Prentice; Julian Parkhill; Nicholas Day; Jeremy Farrar; Gordon Dougan

doi:10.1016/S0147-619X(02)00013-6

Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2

Claire Kidgell, Derek Pickard, John Wain, Keith James, L. T. Diem Nga, To Song Diep, Myron M. Levine, Peadar O'Gaora, Michael B. Prentice, Julian Parkhill, Nicholas Day, Jeremy Farrar, Gordon Dougan

Norwich Medical School

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

pHCM2 is a 106 kbp cryptic plasmid harboured by Salmonella typhi CT18, originally isolated from a typhoid patient in Vietnam. The genome of S. typhi CT18, including pHCM2, has recently been completely sequenced and annotated. Bioinformatic analysis revealed that 57% of the coding sequences (CDSs) encoded on pHCM2 display over 97% DNA sequence identity to the virulence-associated plasmid of Yersinia pestis, pFra. pHCM2 encodes no obvious virulence-associated determinants or antibiotic resistance genes but does encode a wide array of putative genes directly related to DNA metabolism and replication. PCR analysis of a series of S. typhi isolates from Vietnam detected pHCM2-related DNA sequences in some S. typhi isolated before, but not after, 1994. Similar pHCM2-related sequences were also detected in S. typhi isolated from other regions of South East Asia and Pakistan but not elsewhere in the world.

Original language	English
Pages (from-to)	159-171
Number of pages	13
Journal	Plasmid
Volume	47
Issue number	3
DOIs	https://doi.org/10.1016/S0147-619X(02)00013-6
Publication status	Published - May 2002

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10.1016/S0147-619X(02)00013-6

Cite this

@article{8219e3721da44d9892381f0a6136e38e,

title = "Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2",

abstract = "pHCM2 is a 106 kbp cryptic plasmid harboured by Salmonella typhi CT18, originally isolated from a typhoid patient in Vietnam. The genome of S. typhi CT18, including pHCM2, has recently been completely sequenced and annotated. Bioinformatic analysis revealed that 57% of the coding sequences (CDSs) encoded on pHCM2 display over 97% DNA sequence identity to the virulence-associated plasmid of Yersinia pestis, pFra. pHCM2 encodes no obvious virulence-associated determinants or antibiotic resistance genes but does encode a wide array of putative genes directly related to DNA metabolism and replication. PCR analysis of a series of S. typhi isolates from Vietnam detected pHCM2-related DNA sequences in some S. typhi isolated before, but not after, 1994. Similar pHCM2-related sequences were also detected in S. typhi isolated from other regions of South East Asia and Pakistan but not elsewhere in the world.",

author = "Claire Kidgell and Derek Pickard and John Wain and Keith James and Nga, {L. T. Diem} and Diep, {To Song} and Levine, {Myron M.} and Peadar O'Gaora and Prentice, {Michael B.} and Julian Parkhill and Nicholas Day and Jeremy Farrar and Gordon Dougan",

year = "2002",

month = may,

doi = "10.1016/S0147-619X(02)00013-6",

language = "English",

volume = "47",

pages = "159--171",

journal = "Plasmid",

issn = "0147-619X",

publisher = "Academic Press Inc.",

number = "3",

}

Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2. / Kidgell, Claire; Pickard, Derek; Wain, John; James, Keith; Nga, L. T. Diem; Diep, To Song; Levine, Myron M.; O'Gaora, Peadar; Prentice, Michael B.; Parkhill, Julian; Day, Nicholas; Farrar, Jeremy; Dougan, Gordon.

In: Plasmid, Vol. 47, No. 3, 05.2002, p. 159-171.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2

AU - Kidgell, Claire

AU - Pickard, Derek

AU - Wain, John

AU - James, Keith

AU - Nga, L. T. Diem

AU - Diep, To Song

AU - Levine, Myron M.

AU - O'Gaora, Peadar

AU - Prentice, Michael B.

AU - Parkhill, Julian

AU - Day, Nicholas

AU - Farrar, Jeremy

AU - Dougan, Gordon

PY - 2002/5

Y1 - 2002/5

N2 - pHCM2 is a 106 kbp cryptic plasmid harboured by Salmonella typhi CT18, originally isolated from a typhoid patient in Vietnam. The genome of S. typhi CT18, including pHCM2, has recently been completely sequenced and annotated. Bioinformatic analysis revealed that 57% of the coding sequences (CDSs) encoded on pHCM2 display over 97% DNA sequence identity to the virulence-associated plasmid of Yersinia pestis, pFra. pHCM2 encodes no obvious virulence-associated determinants or antibiotic resistance genes but does encode a wide array of putative genes directly related to DNA metabolism and replication. PCR analysis of a series of S. typhi isolates from Vietnam detected pHCM2-related DNA sequences in some S. typhi isolated before, but not after, 1994. Similar pHCM2-related sequences were also detected in S. typhi isolated from other regions of South East Asia and Pakistan but not elsewhere in the world.

AB - pHCM2 is a 106 kbp cryptic plasmid harboured by Salmonella typhi CT18, originally isolated from a typhoid patient in Vietnam. The genome of S. typhi CT18, including pHCM2, has recently been completely sequenced and annotated. Bioinformatic analysis revealed that 57% of the coding sequences (CDSs) encoded on pHCM2 display over 97% DNA sequence identity to the virulence-associated plasmid of Yersinia pestis, pFra. pHCM2 encodes no obvious virulence-associated determinants or antibiotic resistance genes but does encode a wide array of putative genes directly related to DNA metabolism and replication. PCR analysis of a series of S. typhi isolates from Vietnam detected pHCM2-related DNA sequences in some S. typhi isolated before, but not after, 1994. Similar pHCM2-related sequences were also detected in S. typhi isolated from other regions of South East Asia and Pakistan but not elsewhere in the world.

U2 - 10.1016/S0147-619X(02)00013-6

DO - 10.1016/S0147-619X(02)00013-6

M3 - Article

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EP - 171

JO - Plasmid

JF - Plasmid

SN - 0147-619X

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