Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2

Claire Kidgell; Derek Pickard; John Wain; Keith James; L. T. Diem Nga; To Song Diep; Myron M. Levine; Peadar O'Gaora; Michael B. Prentice; Julian Parkhill; Nicholas Day; Jeremy Farrar; Gordon Dougan

doi:10.1016/S0147-619X(02)00013-6

Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2

Claire Kidgell
, Derek Pickard
, John Wain
, Keith James
, L. T. Diem Nga
, To Song Diep
, Myron M. Levine
, Peadar O'Gaora
, Michael B. Prentice
, Julian Parkhill
, Nicholas Day
, Jeremy Farrar
, Gordon Dougan

Norwich Medical School

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

pHCM2 is a 106 kbp cryptic plasmid harboured by Salmonella typhi CT18, originally isolated from a typhoid patient in Vietnam. The genome of S. typhi CT18, including pHCM2, has recently been completely sequenced and annotated. Bioinformatic analysis revealed that 57% of the coding sequences (CDSs) encoded on pHCM2 display over 97% DNA sequence identity to the virulence-associated plasmid of Yersinia pestis, pFra. pHCM2 encodes no obvious virulence-associated determinants or antibiotic resistance genes but does encode a wide array of putative genes directly related to DNA metabolism and replication. PCR analysis of a series of S. typhi isolates from Vietnam detected pHCM2-related DNA sequences in some S. typhi isolated before, but not after, 1994. Similar pHCM2-related sequences were also detected in S. typhi isolated from other regions of South East Asia and Pakistan but not elsewhere in the world.

Original language	English
Pages (from-to)	159-171
Number of pages	13
Journal	Plasmid
Volume	47
Issue number	3
DOIs	https://doi.org/10.1016/S0147-619X(02)00013-6
Publication status	Published - May 2002

Access to Document

10.1016/S0147-619X(02)00013-6

Cite this

@article{8219e3721da44d9892381f0a6136e38e,

title = "Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2",

abstract = "pHCM2 is a 106 kbp cryptic plasmid harboured by Salmonella typhi CT18, originally isolated from a typhoid patient in Vietnam. The genome of S. typhi CT18, including pHCM2, has recently been completely sequenced and annotated. Bioinformatic analysis revealed that 57\% of the coding sequences (CDSs) encoded on pHCM2 display over 97\% DNA sequence identity to the virulence-associated plasmid of Yersinia pestis, pFra. pHCM2 encodes no obvious virulence-associated determinants or antibiotic resistance genes but does encode a wide array of putative genes directly related to DNA metabolism and replication. PCR analysis of a series of S. typhi isolates from Vietnam detected pHCM2-related DNA sequences in some S. typhi isolated before, but not after, 1994. Similar pHCM2-related sequences were also detected in S. typhi isolated from other regions of South East Asia and Pakistan but not elsewhere in the world.",

author = "Claire Kidgell and Derek Pickard and John Wain and Keith James and Nga, \{L. T. Diem\} and Diep, \{To Song\} and Levine, \{Myron M.\} and Peadar O'Gaora and Prentice, \{Michael B.\} and Julian Parkhill and Nicholas Day and Jeremy Farrar and Gordon Dougan",

year = "2002",

month = may,

doi = "10.1016/S0147-619X(02)00013-6",

language = "English",

volume = "47",

pages = "159--171",

journal = "Plasmid",

issn = "1095-9890",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Characterisation and distribution of a cryptic Salmonella typhi plasmid pHCM2

AU - Kidgell, Claire

AU - Pickard, Derek

AU - Wain, John

AU - James, Keith

AU - Nga, L. T. Diem

AU - Diep, To Song

AU - Levine, Myron M.

AU - O'Gaora, Peadar

AU - Prentice, Michael B.

AU - Parkhill, Julian

AU - Day, Nicholas

AU - Farrar, Jeremy

AU - Dougan, Gordon

PY - 2002/5

Y1 - 2002/5

N2 - pHCM2 is a 106 kbp cryptic plasmid harboured by Salmonella typhi CT18, originally isolated from a typhoid patient in Vietnam. The genome of S. typhi CT18, including pHCM2, has recently been completely sequenced and annotated. Bioinformatic analysis revealed that 57% of the coding sequences (CDSs) encoded on pHCM2 display over 97% DNA sequence identity to the virulence-associated plasmid of Yersinia pestis, pFra. pHCM2 encodes no obvious virulence-associated determinants or antibiotic resistance genes but does encode a wide array of putative genes directly related to DNA metabolism and replication. PCR analysis of a series of S. typhi isolates from Vietnam detected pHCM2-related DNA sequences in some S. typhi isolated before, but not after, 1994. Similar pHCM2-related sequences were also detected in S. typhi isolated from other regions of South East Asia and Pakistan but not elsewhere in the world.

AB - pHCM2 is a 106 kbp cryptic plasmid harboured by Salmonella typhi CT18, originally isolated from a typhoid patient in Vietnam. The genome of S. typhi CT18, including pHCM2, has recently been completely sequenced and annotated. Bioinformatic analysis revealed that 57% of the coding sequences (CDSs) encoded on pHCM2 display over 97% DNA sequence identity to the virulence-associated plasmid of Yersinia pestis, pFra. pHCM2 encodes no obvious virulence-associated determinants or antibiotic resistance genes but does encode a wide array of putative genes directly related to DNA metabolism and replication. PCR analysis of a series of S. typhi isolates from Vietnam detected pHCM2-related DNA sequences in some S. typhi isolated before, but not after, 1994. Similar pHCM2-related sequences were also detected in S. typhi isolated from other regions of South East Asia and Pakistan but not elsewhere in the world.

U2 - 10.1016/S0147-619X(02)00013-6

DO - 10.1016/S0147-619X(02)00013-6

M3 - Article

SN - 1095-9890

VL - 47

SP - 159

EP - 171

JO - Plasmid

JF - Plasmid

IS - 3

ER -