TY - JOUR
T1 - Characterisation of genes involved in biosynthesis of coronafacic acid, the polyketide component of the phytotoxin coronatine
AU - Penfold, C. N.
AU - Bender, C. L.
AU - Turner, J. G.
PY - 1996
Y1 - 1996
N2 - Coronafacic acid (CFA) is the polyketide component of coronatine (COR), a phytotoxin produced by the plant pathogen, Pseudomonas syringae. In the present study we have determined the nucleotide sequence of a 3.92-kb DNA fragment involved in CFA biosynthesis. Analysis of the sequence revealed four complete open reading frames (ORFs) designated cfal to cfa4 and one incomplete ORF (cfa5), all transcribed in the same direction. The predicted translation products of cfal, cfa2 and cfa3 showed relatedness to acyl carrier proteins, fatty acid dehydrases and ß-ketoacylsynthases, respectively, which are required for polyketide synthesis. cfal was subcloned, its sequence was confirmed, and it was overexpressed in E. coli to yield a peptide with an apparent molecular mass of 6 kDa.
AB - Coronafacic acid (CFA) is the polyketide component of coronatine (COR), a phytotoxin produced by the plant pathogen, Pseudomonas syringae. In the present study we have determined the nucleotide sequence of a 3.92-kb DNA fragment involved in CFA biosynthesis. Analysis of the sequence revealed four complete open reading frames (ORFs) designated cfal to cfa4 and one incomplete ORF (cfa5), all transcribed in the same direction. The predicted translation products of cfal, cfa2 and cfa3 showed relatedness to acyl carrier proteins, fatty acid dehydrases and ß-ketoacylsynthases, respectively, which are required for polyketide synthesis. cfal was subcloned, its sequence was confirmed, and it was overexpressed in E. coli to yield a peptide with an apparent molecular mass of 6 kDa.
U2 - 10.1016/S0378-1119(96)00550-1
DO - 10.1016/S0378-1119(96)00550-1
M3 - Article
VL - 183
SP - 167
EP - 173
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1-2
ER -