TY - JOUR
T1 - Characterisation of insoluble α-1,3-/α-1,6 mixed linkage glucan produced in addition to soluble α-1,6-linked dextran by glucansucrase (DEX-N) from Leuconostoc citreum ABK-1
AU - Wangpaiboon, Karan
AU - Waiyaseesang, Nisachon
AU - Panpetch, Pawinee
AU - Charoenwongpaiboon, Thanapon
AU - Nepogodiev, Sergey A.
AU - Ekgasit, Sanong
AU - Field, Robert A.
AU - Pichayangkura, Rath
N1 - Funding Information:
This work was supported by a Postdoctoral fellowship from Chulalongkorn University Graduate School (KW). Research at the JIC is supported by the UK BBSRC Institute Strategic Program on Molecules from Nature (MfN) [ BB/PO12523/1 ] and the John Innes Foundation . We also thank Associated Prof. Dr. Kuakarun Krusong, Department of Biochemistry, Faculty of Science, Chulalongkorn University for standard dextrans; Prof. Dr. Siriporn Damrongsakkul, Department of Chemical engineering, Faculty of Engineering, Chulalongkorn University, for the Vibro viscometer SV-10; Dr. Giulia Pergolizzi and Dr. Irina Ivanova, Department of Biological Chemistry, John Innes Centre for chemistry advice; Dr. Paul Brett, Metabolite Analysis Platform, John Innes Centre for assisting with GC-MS analyses, and Ms. Gisma C. Asmarani, Department of Chemistry, Faculty of Science, Chulalongkorn University for assisting the FTIR analysis.
Publisher Copyright:
© 2020
PY - 2020/6/1
Y1 - 2020/6/1
N2 - Glucansucrases catalyse the formation of glucans from sucrose. The glucansucrase-encoding gene from Leuconostoc citreum ABK-1, dex-N, was successfully cloned and expressed in E. coli BL21 Star (DE3). DEX-N produces 2 types of glucans: soluble (S-dextran) and insoluble (I-glucan) glucans. The S-dextran was determined to be ca. 10 kDa in size and contained >90% α-1,6 linkages; along with its water solubility, this is similar to commercial dextran. On the other hand, I-glucan was water-insoluble, harbouring a block-wise pattern of α-1,3 and α-1,6 linkages in its structure. Notably, the FTIR and powder X-ray diffraction pattern of I-glucan exhibited a combination of features found in α-1,6-linked dextran and α-1,3-linked mutan. Although both I-glucan and mutan are insoluble glucans, their physical characteristics are notably dissimilar.
AB - Glucansucrases catalyse the formation of glucans from sucrose. The glucansucrase-encoding gene from Leuconostoc citreum ABK-1, dex-N, was successfully cloned and expressed in E. coli BL21 Star (DE3). DEX-N produces 2 types of glucans: soluble (S-dextran) and insoluble (I-glucan) glucans. The S-dextran was determined to be ca. 10 kDa in size and contained >90% α-1,6 linkages; along with its water solubility, this is similar to commercial dextran. On the other hand, I-glucan was water-insoluble, harbouring a block-wise pattern of α-1,3 and α-1,6 linkages in its structure. Notably, the FTIR and powder X-ray diffraction pattern of I-glucan exhibited a combination of features found in α-1,6-linked dextran and α-1,3-linked mutan. Although both I-glucan and mutan are insoluble glucans, their physical characteristics are notably dissimilar.
KW - Block-wise glucan structure
KW - Glucan polymers
KW - Glucansucrase
KW - Glucosyltransferase-SI
KW - Leuconostoc citreum
UR - http://www.scopus.com/inward/record.url?scp=85080142946&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2020.02.247
DO - 10.1016/j.ijbiomac.2020.02.247
M3 - Article
C2 - 32097735
AN - SCOPUS:85080142946
VL - 152
SP - 473
EP - 482
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
SN - 0141-8130
ER -