Characterization of atypical scrapie cases from Great Britain in transgenic ovine PrP mice

Peter C. Griffiths; John Spiropoulos; Richard Lockey; Anna C. Tout; Dhanushka Jayasena; Jane M. Plater; Alun Chave; Robert B. Green; Sarah Simonini; Leigh Thorne; Ian Dexter; Anne Balkema-Buschmann; Martin H. Groschup; Vincent Béringue; Annick Le Dur; Hubert Laude; James Hope

doi:10.1099/vir.0.018986-0

Characterization of atypical scrapie cases from Great Britain in transgenic ovine PrP mice

Peter C. Griffiths
, John Spiropoulos
, Richard Lockey
, Anna C. Tout
, Dhanushka Jayasena
, Jane M. Plater
, Alun Chave
, Robert B. Green
, Sarah Simonini
, Leigh Thorne
, Ian Dexter
, Anne Balkema-Buschmann
, Martin H. Groschup
, Vincent Béringue
, Annick Le Dur
, Hubert Laude
, James Hope

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Twenty-four atypical scrapie cases from sheep with different prion protein genotypes from Great Britain were transmitted to transgenic tg338 and/or TgshpXI mice expressing sheep PrP alleles, but failed to transmit to wild-type mice. Mean incubation periods were 200-300 days in tg338 mice and 300-500 days in TgshpXI mice. Survival times in C57BL/6 and VM/Dk mice were >700 days. Western blot analysis of mouse brain samples revealed similar multi-band, protease-resistant prion protein (PrP^res) profiles, including an unglycosylated band at ∼8-11 kDa, which was shown by antibody mapping to correspond to the ∼93-148 aa portion of the PrP molecule. In transgenic mice, the incubation periods, Western blot PrP^res profiles, brain lesion profiles and abnormal PrP (PrP^Sc) distribution patterns produced by the Great Britain atypical scrapie isolates were similar and compatible with the biological characteristics of other European atypical scrapie or Nor98 cases.

Original language	English
Pages (from-to)	2132-2138
Number of pages	7
Journal	Journal of General Virology
Volume	91
Issue number	8
DOIs	https://doi.org/10.1099/vir.0.018986-0
Publication status	Published - Aug 2010

Access to Document

10.1099/vir.0.018986-0

Cite this

Griffiths, P. C., Spiropoulos, J., Lockey, R., Tout, A. C., Jayasena, D., Plater, J. M., Chave, A., Green, R. B., Simonini, S., Thorne, L., Dexter, I., Balkema-Buschmann, A., Groschup, M. H., Béringue, V., Le Dur, A., Laude, H., & Hope, J. (2010). Characterization of atypical scrapie cases from Great Britain in transgenic ovine PrP mice. Journal of General Virology, 91(8), 2132-2138. https://doi.org/10.1099/vir.0.018986-0

@article{51cdebd9cc3640a0b4fe90f4603fd82b,

title = "Characterization of atypical scrapie cases from Great Britain in transgenic ovine PrP mice",

abstract = "Twenty-four atypical scrapie cases from sheep with different prion protein genotypes from Great Britain were transmitted to transgenic tg338 and/or TgshpXI mice expressing sheep PrP alleles, but failed to transmit to wild-type mice. Mean incubation periods were 200-300 days in tg338 mice and 300-500 days in TgshpXI mice. Survival times in C57BL/6 and VM/Dk mice were >700 days. Western blot analysis of mouse brain samples revealed similar multi-band, protease-resistant prion protein (PrPres) profiles, including an unglycosylated band at ∼8-11 kDa, which was shown by antibody mapping to correspond to the ∼93-148 aa portion of the PrP molecule. In transgenic mice, the incubation periods, Western blot PrPres profiles, brain lesion profiles and abnormal PrP (PrPSc) distribution patterns produced by the Great Britain atypical scrapie isolates were similar and compatible with the biological characteristics of other European atypical scrapie or Nor98 cases.",

author = "Griffiths, \{Peter C.\} and John Spiropoulos and Richard Lockey and Tout, \{Anna C.\} and Dhanushka Jayasena and Plater, \{Jane M.\} and Alun Chave and Green, \{Robert B.\} and Sarah Simonini and Leigh Thorne and Ian Dexter and Anne Balkema-Buschmann and Groschup, \{Martin H.\} and Vincent B{\'e}ringue and \{Le Dur\}, Annick and Hubert Laude and James Hope",

year = "2010",

month = aug,

doi = "10.1099/vir.0.018986-0",

language = "English",

volume = "91",

pages = "2132--2138",

journal = "Journal of General Virology",

issn = "0022-1317",

publisher = "Microbiology Society",

number = "8",

}

Griffiths, PC, Spiropoulos, J, Lockey, R, Tout, AC, Jayasena, D, Plater, JM, Chave, A, Green, RB, Simonini, S, Thorne, L, Dexter, I, Balkema-Buschmann, A, Groschup, MH, Béringue, V, Le Dur, A, Laude, H & Hope, J 2010, 'Characterization of atypical scrapie cases from Great Britain in transgenic ovine PrP mice', Journal of General Virology, vol. 91, no. 8, pp. 2132-2138. https://doi.org/10.1099/vir.0.018986-0

TY - JOUR

T1 - Characterization of atypical scrapie cases from Great Britain in transgenic ovine PrP mice

AU - Griffiths, Peter C.

AU - Spiropoulos, John

AU - Lockey, Richard

AU - Tout, Anna C.

AU - Jayasena, Dhanushka

AU - Plater, Jane M.

AU - Chave, Alun

AU - Green, Robert B.

AU - Simonini, Sarah

AU - Thorne, Leigh

AU - Dexter, Ian

AU - Balkema-Buschmann, Anne

AU - Groschup, Martin H.

AU - Béringue, Vincent

AU - Le Dur, Annick

AU - Laude, Hubert

AU - Hope, James

PY - 2010/8

Y1 - 2010/8

N2 - Twenty-four atypical scrapie cases from sheep with different prion protein genotypes from Great Britain were transmitted to transgenic tg338 and/or TgshpXI mice expressing sheep PrP alleles, but failed to transmit to wild-type mice. Mean incubation periods were 200-300 days in tg338 mice and 300-500 days in TgshpXI mice. Survival times in C57BL/6 and VM/Dk mice were >700 days. Western blot analysis of mouse brain samples revealed similar multi-band, protease-resistant prion protein (PrPres) profiles, including an unglycosylated band at ∼8-11 kDa, which was shown by antibody mapping to correspond to the ∼93-148 aa portion of the PrP molecule. In transgenic mice, the incubation periods, Western blot PrPres profiles, brain lesion profiles and abnormal PrP (PrPSc) distribution patterns produced by the Great Britain atypical scrapie isolates were similar and compatible with the biological characteristics of other European atypical scrapie or Nor98 cases.

AB - Twenty-four atypical scrapie cases from sheep with different prion protein genotypes from Great Britain were transmitted to transgenic tg338 and/or TgshpXI mice expressing sheep PrP alleles, but failed to transmit to wild-type mice. Mean incubation periods were 200-300 days in tg338 mice and 300-500 days in TgshpXI mice. Survival times in C57BL/6 and VM/Dk mice were >700 days. Western blot analysis of mouse brain samples revealed similar multi-band, protease-resistant prion protein (PrPres) profiles, including an unglycosylated band at ∼8-11 kDa, which was shown by antibody mapping to correspond to the ∼93-148 aa portion of the PrP molecule. In transgenic mice, the incubation periods, Western blot PrPres profiles, brain lesion profiles and abnormal PrP (PrPSc) distribution patterns produced by the Great Britain atypical scrapie isolates were similar and compatible with the biological characteristics of other European atypical scrapie or Nor98 cases.

UR - https://www.scopus.com/pages/publications/77955291479

U2 - 10.1099/vir.0.018986-0

DO - 10.1099/vir.0.018986-0

M3 - Article

C2 - 20392900

AN - SCOPUS:77955291479

SN - 0022-1317

VL - 91

SP - 2132

EP - 2138

JO - Journal of General Virology

JF - Journal of General Virology

IS - 8

ER -

Characterization of atypical scrapie cases from Great Britain in transgenic ovine PrP mice

Abstract

Access to Document

Other files and links

Cite this