Characterization of cell wall components of wheat straw following hydrothermal pretreatment and fractionation

Zara Merali, Justin D. Ho, Samuel R. A. Collins, Gwénaëlle Le Gall, Adam Elliston, Andres Kaesper, Keith W. Waldron

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52 Citations (Scopus)

Abstract

Thermophysical pretreatment enhances the enzymatic hydrolysis of lignocellulose. However, its impact on cell wall chemistry is still poorly understood. This paper reports the effects of hydrothermal pretreatment on the degradation and alkali-extractability of wheat straw cell wall polymers. Pretreatment resulted in loss and/or solubilization of arabinoxylans (by 53%), ferulic and diferulic acids which are important cross-linking agents accompanied by concomitant increases in cellulose (up to 43%) and lignin (29%). The remaining water-insoluble hemicelluloses were more readily extractable in alkali and were reduced in molecular weight indicating substantial thermochemical depolymerization. They were also associated with smaller but significant amounts of (cellulose-derived) glucose. The alkali-insoluble residues consisted predominantly of cellulosic glucose and lignin and contained p-coumaric acid. The depolymerization of hemicelluloses, reduction in cinnamic acids and partial degradation of cellulose is likely to contribute significantly to the accessibility of cellulases during subsequent enzymolysis. (C) 2012 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)226-234
Number of pages9
JournalBioresource Technology
Volume131
DOIs
Publication statusPublished - Mar 2013
Externally publishedYes

Keywords

  • Cellulose
  • Fractionation
  • Phenolics
  • Hydrothermal pretreatment
  • Wheat straw

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