Characterization of Human Transforming Genes from Chemically Transformed, Teratocarcinoma, and Pancreatic Carcinoma Cell Lines

Colin S. Cooper; Donald G. Blair; Marianne K. Oskarsson; Michael A. Tainsky; Lou A. Eader; George F. Vande Woude

Characterization of Human Transforming Genes from Chemically Transformed, Teratocarcinoma, and Pancreatic Carcinoma Cell Lines

Colin S. Cooper, Donald G. Blair, Marianne K. Oskarsson, Michael A. Tainsky, Lou A. Eader, George F. Vande Woude

Research output: Contribution to journal › Article › peer-review

80 Citations (Scopus)

Abstract

Dominant transforming genes that were transferred to mouse N1H3T3 cells by cellular DNAs prepared from a chemically transformed human cell line (MNNG-HOS), a human teratocarcinoma cell line (PA1), and a human pancreatic carcinoma cell line (A1165) were characterized (a) analyzing the repetitive human DNA sequences that were associated with the transforming gene and (b) determining their relationship to the oncogenes of the Harvey (ras”) and Kirsten (rasK) sarcoma viruses and to the human neuroblastoma transforming gene (ras”). The results show that the transforming gene activated in the teratocarcinoma cell line is identical to the neuroblastoma transforming gene and that the transforming gene of the pancreatic carcinoma cell line is a human homologue of rask. In contrast, the transforming gene activated in the chemically transformed human cell line showed no detectable homology to rasK, rasH, and rasN.

Original language	English
Pages (from-to)	1-10
Number of pages	10
Journal	Cancer Research
Volume	44
Issue number	1
Publication status	Published - 1 Jan 1984

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title = "Characterization of Human Transforming Genes from Chemically Transformed, Teratocarcinoma, and Pancreatic Carcinoma Cell Lines",

abstract = "Dominant transforming genes that were transferred to mouse N1H3T3 cells by cellular DNAs prepared from a chemically transformed human cell line (MNNG-HOS), a human teratocarcinoma cell line (PA1), and a human pancreatic carcinoma cell line (A1165) were characterized (a) analyzing the repetitive human DNA sequences that were associated with the transforming gene and (b) determining their relationship to the oncogenes of the Harvey (ras”) and Kirsten (rasK) sarcoma viruses and to the human neuroblastoma transforming gene (ras”). The results show that the transforming gene activated in the teratocarcinoma cell line is identical to the neuroblastoma transforming gene and that the transforming gene of the pancreatic carcinoma cell line is a human homologue of rask. In contrast, the transforming gene activated in the chemically transformed human cell line showed no detectable homology to rasK, rasH, and rasN.",

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T1 - Characterization of Human Transforming Genes from Chemically Transformed, Teratocarcinoma, and Pancreatic Carcinoma Cell Lines

AU - Cooper, Colin S.

AU - Blair, Donald G.

AU - Oskarsson, Marianne K.

AU - Tainsky, Michael A.

AU - Eader, Lou A.

AU - Vande Woude, George F.

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N2 - Dominant transforming genes that were transferred to mouse N1H3T3 cells by cellular DNAs prepared from a chemically transformed human cell line (MNNG-HOS), a human teratocarcinoma cell line (PA1), and a human pancreatic carcinoma cell line (A1165) were characterized (a) analyzing the repetitive human DNA sequences that were associated with the transforming gene and (b) determining their relationship to the oncogenes of the Harvey (ras”) and Kirsten (rasK) sarcoma viruses and to the human neuroblastoma transforming gene (ras”). The results show that the transforming gene activated in the teratocarcinoma cell line is identical to the neuroblastoma transforming gene and that the transforming gene of the pancreatic carcinoma cell line is a human homologue of rask. In contrast, the transforming gene activated in the chemically transformed human cell line showed no detectable homology to rasK, rasH, and rasN.

AB - Dominant transforming genes that were transferred to mouse N1H3T3 cells by cellular DNAs prepared from a chemically transformed human cell line (MNNG-HOS), a human teratocarcinoma cell line (PA1), and a human pancreatic carcinoma cell line (A1165) were characterized (a) analyzing the repetitive human DNA sequences that were associated with the transforming gene and (b) determining their relationship to the oncogenes of the Harvey (ras”) and Kirsten (rasK) sarcoma viruses and to the human neuroblastoma transforming gene (ras”). The results show that the transforming gene activated in the teratocarcinoma cell line is identical to the neuroblastoma transforming gene and that the transforming gene of the pancreatic carcinoma cell line is a human homologue of rask. In contrast, the transforming gene activated in the chemically transformed human cell line showed no detectable homology to rasK, rasH, and rasN.

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Characterization of Human Transforming Genes from Chemically Transformed, Teratocarcinoma, and Pancreatic Carcinoma Cell Lines

Abstract

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