Characterization of tetranucleotide microsatellite loci in a Lake Victorian, haplochromine cichlid fish: A Pundamilia pundamilia x Pundamilia nyererei hybrid

Martin I. Taylor, Fiona Meardon, George Turner, Ole Seehausen, Hilary D. J. Mrosso, Ciro Rico

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Abstract

The haplochromine cichlid fishes inhabiting Lake Victoria in East Africa are of great interest to evolutionary biologists. We have isolated and optimized six tetranucleotide and a single dinucleotide locus from a hybrid of the haplochromine cichlid fishes, Pundamilia pundamilia and P. nyererei. Characterization in 18 individuals of P. nyererei from a single wild population revealed between six and 18 alleles per locus, with expected heterozygosity ranging from 0.68 to 0.94. These loci will prove useful for investigations of population structure, and elucidating relationships between closely related species. An additional 26 unoptimized loci have been deposited with GenBank and the MEN database.
Original languageEnglish
Pages (from-to)443-445
Number of pages3
JournalMolecular Ecology Notes
Volume2
Issue number4
Early online date17 Oct 2002
DOIs
Publication statusPublished - Dec 2002

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