Characterization of the mouse met proto-oncogene

A. M.L. Chan, H. W.S. King, E. A. Deakin, P. R. Tempest, J. Hilkens, V. Kroezen, D. R. Edwards, A. J. Wills, P. Brookes, C. S. Cooper

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The DNA sequence of cDNA clones prepared from transcripts of the mouse met proto-oncogene reveals that the mouse met gene encodes a 1380 amino acid protein with the characteristics of a growth factor receptor. This protein can be divided into several putative domains, including an intracellular protein tyrosine kinase domain, a transmembrane domain and a 929 amino acid extracellular domain, possessing a potential proteolytic cleavage site with the sequence Lys-Arg-Arg-Lys-Arg-Ser. To gain additional insights into the function of the met protein we have examined the level of met transcripts in tissues of the late-gestation mouse conceptus. Transcription of met was observed in most of the tissues analysed, but the highest levels of met mRNA were detected in the yolk sac, amnion and kidney; no transcripts were detectable in the calvaria. Chromosomal localization using a series of mouse-hamster hybrid cell lines has demonstrated that met is located on mouse chromosome 6.

Original languageEnglish
Pages (from-to)593-599
Number of pages7
Issue number6
Publication statusPublished - 1988

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