Characterization of two non-locus of enterocyte effacement-encoded type III-translocated effectors, NleC and NleD, in attaching and effacing pathogens

Olivier Marches; Siouxsie Wiles; Francis Dziva; Roberto M. La Ragione; Stephanie Schuller; Angus Best; Alan D. Phillips; Elizabeth L. Hartland; Martin J. Woodward; Mark P. Stevens; Gad Frankel

doi:10.1128/IAI.73.12.8411-8417.2005

Characterization of two non-locus of enterocyte effacement-encoded type III-translocated effectors, NleC and NleD, in attaching and effacing pathogens

Olivier Marches, Siouxsie Wiles, Francis Dziva, Roberto M. La Ragione, Stephanie Schuller, Angus Best, Alan D. Phillips, Elizabeth L. Hartland, Martin J. Woodward, Mark P. Stevens, Gad Frankel

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54 Citations (Scopus)

Abstract

Intestinal colonization by enteropathogenic and enterohemorrhagic Escherichia coli requires the locus of enterocyte effacement-encoded type III secretion system. We report that NleC and NleD are translocated into host cells via this system. Deletion mutants induced attaching and effacing lesions in vitro, while infection of calves or lambs showed that neither gene was required for colonization.

Original language	English
Pages (from-to)	8411-8417
Number of pages	7
Journal	Infection and Immunity
Volume	73
Issue number	12
DOIs	https://doi.org/10.1128/IAI.73.12.8411-8417.2005
Publication status	Published - Dec 2005

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10.1128/IAI.73.12.8411-8417.2005

Cite this

Marches, O., Wiles, S., Dziva, F., La Ragione, R. M., Schuller, S., Best, A., Phillips, A. D., Hartland, E. L., Woodward, M. J., Stevens, M. P., & Frankel, G. (2005). Characterization of two non-locus of enterocyte effacement-encoded type III-translocated effectors, NleC and NleD, in attaching and effacing pathogens. Infection and Immunity, 73(12), 8411-8417. https://doi.org/10.1128/IAI.73.12.8411-8417.2005

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title = "Characterization of two non-locus of enterocyte effacement-encoded type III-translocated effectors, NleC and NleD, in attaching and effacing pathogens",

abstract = "Intestinal colonization by enteropathogenic and enterohemorrhagic Escherichia coli requires the locus of enterocyte effacement-encoded type III secretion system. We report that NleC and NleD are translocated into host cells via this system. Deletion mutants induced attaching and effacing lesions in vitro, while infection of calves or lambs showed that neither gene was required for colonization.",

author = "Olivier Marches and Siouxsie Wiles and Francis Dziva and {La Ragione}, {Roberto M.} and Stephanie Schuller and Angus Best and Phillips, {Alan D.} and Hartland, {Elizabeth L.} and Woodward, {Martin J.} and Stevens, {Mark P.} and Gad Frankel",

year = "2005",

month = dec,

doi = "10.1128/IAI.73.12.8411-8417.2005",

language = "English",

volume = "73",

pages = "8411--8417",

journal = "Infection and Immunity",

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publisher = "American Society for Microbiology",

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Marches, O, Wiles, S, Dziva, F, La Ragione, RM, Schuller, S, Best, A, Phillips, AD, Hartland, EL, Woodward, MJ, Stevens, MP & Frankel, G 2005, 'Characterization of two non-locus of enterocyte effacement-encoded type III-translocated effectors, NleC and NleD, in attaching and effacing pathogens', Infection and Immunity, vol. 73, no. 12, pp. 8411-8417. https://doi.org/10.1128/IAI.73.12.8411-8417.2005

TY - JOUR

T1 - Characterization of two non-locus of enterocyte effacement-encoded type III-translocated effectors, NleC and NleD, in attaching and effacing pathogens

AU - Marches, Olivier

AU - Wiles, Siouxsie

AU - Dziva, Francis

AU - La Ragione, Roberto M.

AU - Schuller, Stephanie

AU - Best, Angus

AU - Phillips, Alan D.

AU - Hartland, Elizabeth L.

AU - Woodward, Martin J.

AU - Stevens, Mark P.

AU - Frankel, Gad

PY - 2005/12

Y1 - 2005/12

N2 - Intestinal colonization by enteropathogenic and enterohemorrhagic Escherichia coli requires the locus of enterocyte effacement-encoded type III secretion system. We report that NleC and NleD are translocated into host cells via this system. Deletion mutants induced attaching and effacing lesions in vitro, while infection of calves or lambs showed that neither gene was required for colonization.

AB - Intestinal colonization by enteropathogenic and enterohemorrhagic Escherichia coli requires the locus of enterocyte effacement-encoded type III secretion system. We report that NleC and NleD are translocated into host cells via this system. Deletion mutants induced attaching and effacing lesions in vitro, while infection of calves or lambs showed that neither gene was required for colonization.

U2 - 10.1128/IAI.73.12.8411-8417.2005

DO - 10.1128/IAI.73.12.8411-8417.2005

M3 - Article

VL - 73

SP - 8411

EP - 8417

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 12

ER -