Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection

Rita Sousa-Nunes, Amer Ahmed Rana, Ross Kettleborough, Joshua M. Brickman, Melanie Clements, Alistair Forrest, Sean Grimmond, Philip Avner, James C. Smith, Sally L. Dunwoodie, Rosa S. P. Beddington

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24 Citations (Scopus)


This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.

Original languageEnglish
Pages (from-to)2609-2620
Number of pages12
JournalGenome Research
Issue number12
Publication statusPublished - Dec 2003

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