Chitin perception in plasmodesmata characterizes submembrane immune-signaling specificity in plants

Cécilia Cheval, Sebastian Samwald, Matthew G. Johnston, Jeroen de Keijzer, Andrew Breakspear, Xiaokun Liu, Annalisa Bellandi, Yasuhiro Kadota, Cyril Zipfel, Christine Faulkner

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)
7 Downloads (Pure)

Abstract

The plasma membrane (PM) is composed of heterogeneous subdomains, characterized by differences in protein and lipid composition. PM receptors can be dynamically sorted into membrane domains to underpin signaling in response to extracellular stimuli. In plants, the plasmodesmal PM is a discrete microdomain that hosts specific receptors and responses. We exploited the independence of this PM domain to investigate how membrane domains can independently integrate a signal that triggers responses across the cell. Focusing on chitin signaling, we found that responses in the plasmodesmal PM require the LysM receptor kinases LYK4 and LYK5 in addition to LYM2. Chitin induces dynamic changes in the localization, association, or mobility of these receptors, but only LYM2 and LYK4 are detected in the plasmodesmal PM. We further uncovered that chitin-induced production of reactive oxygen species and callose depends on specific signaling events that lead to plasmodesmata closure. Our results demonstrate that distinct membrane domains can integrate a common signal with specific machinery that initiates discrete signaling cascades to produce a localized response.

Original languageEnglish
Pages (from-to)9621-9629
Number of pages9
JournalProceedings of the National Academy of Sciences of the United States of America
Volume117
Issue number17
Early online date13 Apr 2020
DOIs
Publication statusPublished - 28 Apr 2020

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