TY - JOUR
T1 - Chromatin accessibility landscapes activated by cell-surface and intracellular immune receptors
AU - Ding, Pingtao
AU - Sakai, Toshiyuki
AU - Shrestha, Ram Krishna
AU - Perez, Nicolas Manosalva
AU - Guo, Wenbin
AU - Ngou, Bruno Pok Man
AU - He, Shengbo
AU - Liu, Chang
AU - Feng, Xiaoqi
AU - Zhang, Runxuan
AU - Vandepoele, Klaas
AU - MacLean, Dan
AU - Jones, Jonathan D. G.
PY - 2021/12/4
Y1 - 2021/12/4
N2 - Activation of cell-surface and intracellular receptor-mediated immunity results in rapid transcriptional reprogramming that underpins disease resistance. However, the mechanisms by which co-activation of both immune systems lead to transcriptional changes are not clear. Here, we combine RNA-seq and ATAC-seq to define changes in gene expression and chromatin accessibility. Activation of cell-surface or intracellular receptor-mediated immunity, or both, increases chromatin accessibility at induced defence genes. Analysis of ATAC-seq and RNA-seq data combined with publicly available information on transcription factor DNA-binding motifs enabled comparison of individual gene regulatory networks activated by cell-surface or intracellular receptor-mediated immunity, or by both. These results and analyses reveal overlapping and conserved transcriptional regulatory mechanisms between the two immune systems.
AB - Activation of cell-surface and intracellular receptor-mediated immunity results in rapid transcriptional reprogramming that underpins disease resistance. However, the mechanisms by which co-activation of both immune systems lead to transcriptional changes are not clear. Here, we combine RNA-seq and ATAC-seq to define changes in gene expression and chromatin accessibility. Activation of cell-surface or intracellular receptor-mediated immunity, or both, increases chromatin accessibility at induced defence genes. Analysis of ATAC-seq and RNA-seq data combined with publicly available information on transcription factor DNA-binding motifs enabled comparison of individual gene regulatory networks activated by cell-surface or intracellular receptor-mediated immunity, or by both. These results and analyses reveal overlapping and conserved transcriptional regulatory mechanisms between the two immune systems.
KW - ATAC-seq and RNA-seq
KW - PRR and NLR
KW - cell surface immune receptors
KW - chromatin accessibility
KW - gene regulatory network
KW - intracellular immune receptors
KW - plant innate immunity
KW - transcriptional regulation
UR - http://www.scopus.com/inward/record.url?scp=85115169559&partnerID=8YFLogxK
U2 - 10.1093/jxb/erab373
DO - 10.1093/jxb/erab373
M3 - Article
SN - 0022-0957
VL - 72
SP - 7927
EP - 7941
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
IS - 22
ER -