Chronic microsensors for longitudinal, subsecond dopamine detection in behaving animals

Jeremy J Clark, Stefan G Sandberg, Matthew J Wanat, Jerylin O Gan, Eric A Horne, Andrew S Hart, Christina A Akers, Jones G Parker, Ingo Willuhn, Vicente Martinez, Scott B Evans, Nephi Stella, Paul E M Phillips

Research output: Contribution to journalArticlepeer-review

240 Citations (Scopus)

Abstract

Neurotransmission operates on a millisecond timescale but is changed by normal experience or neuropathology over days to months. Despite the importance of long-term neurotransmitter dynamics, no technique exists to track these changes in a subject from day to day over extended periods of time. Here we describe and characterize a microsensor that can detect the neurotransmitter dopamine with subsecond temporal resolution over months in vivo in rats and mice.
Original languageEnglish
Pages (from-to)126-9
Number of pages4
JournalNature Methods
Volume7
Issue number2
DOIs
Publication statusPublished - Feb 2010

Keywords

  • Animals
  • Behavior, Animal
  • Biosensing Techniques
  • Dopamine
  • Electrochemistry
  • Electrodes, Implanted
  • Equipment Design
  • Equipment Failure Analysis
  • Mesencephalon
  • Mice
  • Microelectrodes
  • Miniaturization
  • Monitoring, Ambulatory
  • Neurotransmitter Agents
  • Rats
  • Reproducibility of Results
  • Sensitivity and Specificity

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