Chronic microsensors for longitudinal, subsecond dopamine detection in behaving animals

Jeremy J Clark; Stefan G Sandberg; Matthew J Wanat; Jerylin O Gan; Eric A Horne; Andrew S Hart; Christina A Akers; Jones G Parker; Ingo Willuhn; Vicente Martinez; Scott B Evans; Nephi Stella; Paul E M Phillips

doi:10.1038/nmeth.1412

Chronic microsensors for longitudinal, subsecond dopamine detection in behaving animals

Jeremy J Clark, Stefan G Sandberg, Matthew J Wanat, Jerylin O Gan, Eric A Horne, Andrew S Hart, Christina A Akers, Jones G Parker, Ingo Willuhn, Vicente Martinez, Scott B Evans, Nephi Stella, Paul E M Phillips

Research output: Contribution to journal › Article › peer-review

295 Citations (SciVal)

Abstract

Neurotransmission operates on a millisecond timescale but is changed by normal experience or neuropathology over days to months. Despite the importance of long-term neurotransmitter dynamics, no technique exists to track these changes in a subject from day to day over extended periods of time. Here we describe and characterize a microsensor that can detect the neurotransmitter dopamine with subsecond temporal resolution over months in vivo in rats and mice.

Original language	English
Pages (from-to)	126-9
Number of pages	4
Journal	Nature Methods
Volume	7
Issue number	2
DOIs	https://doi.org/10.1038/nmeth.1412
Publication status	Published - Feb 2010

Keywords

Animals
Behavior, Animal
Biosensing Techniques
Dopamine
Electrochemistry
Electrodes, Implanted
Equipment Design
Equipment Failure Analysis
Mesencephalon
Mice
Microelectrodes
Miniaturization
Monitoring, Ambulatory
Neurotransmitter Agents
Rats
Reproducibility of Results
Sensitivity and Specificity

Access to Document

10.1038/nmeth.1412

Cite this

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abstract = "Neurotransmission operates on a millisecond timescale but is changed by normal experience or neuropathology over days to months. Despite the importance of long-term neurotransmitter dynamics, no technique exists to track these changes in a subject from day to day over extended periods of time. Here we describe and characterize a microsensor that can detect the neurotransmitter dopamine with subsecond temporal resolution over months in vivo in rats and mice.",

keywords = "Animals, Behavior, Animal, Biosensing Techniques, Dopamine, Electrochemistry, Electrodes, Implanted, Equipment Design, Equipment Failure Analysis, Mesencephalon, Mice, Microelectrodes, Miniaturization, Monitoring, Ambulatory, Neurotransmitter Agents, Rats, Reproducibility of Results, Sensitivity and Specificity",

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AU - Clark, Jeremy J

AU - Sandberg, Stefan G

AU - Wanat, Matthew J

AU - Gan, Jerylin O

AU - Horne, Eric A

AU - Hart, Andrew S

AU - Akers, Christina A

AU - Parker, Jones G

AU - Willuhn, Ingo

AU - Martinez, Vicente

AU - Evans, Scott B

AU - Stella, Nephi

AU - Phillips, Paul E M

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KW - Behavior, Animal

KW - Biosensing Techniques

KW - Dopamine

KW - Electrochemistry

KW - Electrodes, Implanted

KW - Equipment Design

KW - Equipment Failure Analysis

KW - Mesencephalon

KW - Mice

KW - Microelectrodes

KW - Miniaturization

KW - Monitoring, Ambulatory

KW - Neurotransmitter Agents

KW - Rats

KW - Reproducibility of Results

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JO - Nature Methods

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