Clinically compatible advances in blood-derived endothelial progenitor cell isolation and reprogramming for translational applications

Sarah Eminli, Baraa Kwieder, Kevin Yi, Christopher J.Z. Huang, Jung Il Moon, C. Hong Chang, Fedir N. Kiskin, Nicholas W. Morrell, Brad Hamilton, Amer A. Rana

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The promise of using induced pluripotent stem cells (iPSCs) for cellular therapies has been hampered by the lack of easily isolatable and well characterized source cells whose genomes have undergone minimal changes during their processing. Blood-derived late-outgrowth endothelial progenitor cells (EPCs) are used for disease modeling and have potential therapeutic uses including cell transplantation and the translation of induced pluripotent stem cell (iPSC) derivatives. However, the current isolation of EPCs has been inconsistent and requires at least 40−80 mL of blood, limiting their wider use. In addition, previous EPC reprogramming methods precluded the translation of EPC-derived iPSCs to the clinic. Here a series of clinically-compatible advances in the isolation and reprogramming of EPCs is presented, including a reduction of blood sampling volumes to 10 mL and use of highly efficient RNA-based reprogramming methods together with autologous human serum, resulting in clinically relevant iPSCs carrying minimal copy number variations (CNVs) compared to their parent line.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalNew Biotechnology
Volume63
Early online date12 Feb 2021
DOIs
Publication statusPublished - 25 Jul 2021

Keywords

  • Clinical translation
  • EPC
  • iPSC
  • Reprogramming
  • Self-replicating mRNA

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