Cloning and characterization of gentamicin-resistance genes from Micromonospora purpurea and Micromonospora rosea

Gabriella H. Kelemen, Eric Cundliffe, István Financsek

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41 Citations (Scopus)


Aminoglycoside-resistance genes (grm) were cloned from a gentamicin producer Micromonospora purpurea and a sisomicin producer Micromonospora rosea. The nucleotide (nt) sequences of both genes were determined and the similarity between them was very high (90.4% identity). In either case, the transcription start point was localised to about 11 nt upstream from the likely translation start codons of grm, which is expressed as a polycistronic transcript. In studies to be reported elsewhere, it has been established that the M. purpurea grm gene encodes a ribosomal RNA methyltransferase. Here, we confirmed that the similarity of the two genes exists not only at the structural but also at the functional level.

Original languageEnglish
Pages (from-to)53-60
Number of pages8
Issue number1
Publication statusPublished - 1 Feb 1991


  • aminoglycoside-resistance
  • Recombinant DNA
  • ribosomal RNA methylase
  • transcript mapping

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