Cloning and sequencing of ovine Flt3 ligand

S. S. M. Chan; N. Lyons; I. McConnell; B. A. Blacklaws

doi:10.1111/j.1744-313X.2007.00659.x

Cloning and sequencing of ovine Flt3 ligand

S. S. M. Chan (Lead Author), N. Lyons, I. McConnell, B. A. Blacklaws

Research output: Contribution to journal › Article › peer-review

Abstract

A cDNA (879 bp) containing the complete open reading frame of ovine Fms-related tyrosine kinase 3 ligand (Flt3-L) was amplified by reverse transcriptase polymerase chain reaction (RT-PCR), cloned and sequenced. The deduced amino acid sequence has 97.6% similarity with bovine Flt3-L isoform 1 and shares the long cytoplasmic domain observed in bovine Flt3-L but not in human Flt3-L.

Original language	English
Pages (from-to)	167-171
Number of pages	5
Journal	International Journal of Immunogenetics
Volume	34
Issue number	3
Early online date	7 Mar 2007
DOIs	https://doi.org/10.1111/j.1744-313X.2007.00659.x
Publication status	Published - Jun 2007

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10.1111/j.1744-313X.2007.00659.x

Cite this

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title = "Cloning and sequencing of ovine Flt3 ligand",

abstract = "A cDNA (879 bp) containing the complete open reading frame of ovine Fms-related tyrosine kinase 3 ligand (Flt3-L) was amplified by reverse transcriptase polymerase chain reaction (RT-PCR), cloned and sequenced. The deduced amino acid sequence has 97.6% similarity with bovine Flt3-L isoform 1 and shares the long cytoplasmic domain observed in bovine Flt3-L but not in human Flt3-L.",

author = "Chan, {S. S. M.} and N. Lyons and I. McConnell and Blacklaws, {B. A.}",

note = "Funding information: Nicholas Lyons was supported by a Durham Award, King's College, Cambridge. Simon Chan was supported by a BBSRC PhD studentship. Barbara Blacklaws was supported by Wellcome Trust Grant 060000/2/99/2. ",

year = "2007",

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doi = "10.1111/j.1744-313X.2007.00659.x",

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journal = "International Journal of Immunogenetics",

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AU - Chan, S. S. M.

AU - Lyons, N.

AU - McConnell, I.

AU - Blacklaws, B. A.

N1 - Funding information: Nicholas Lyons was supported by a Durham Award, King's College, Cambridge. Simon Chan was supported by a BBSRC PhD studentship. Barbara Blacklaws was supported by Wellcome Trust Grant 060000/2/99/2.

PY - 2007/6

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N2 - A cDNA (879 bp) containing the complete open reading frame of ovine Fms-related tyrosine kinase 3 ligand (Flt3-L) was amplified by reverse transcriptase polymerase chain reaction (RT-PCR), cloned and sequenced. The deduced amino acid sequence has 97.6% similarity with bovine Flt3-L isoform 1 and shares the long cytoplasmic domain observed in bovine Flt3-L but not in human Flt3-L.

AB - A cDNA (879 bp) containing the complete open reading frame of ovine Fms-related tyrosine kinase 3 ligand (Flt3-L) was amplified by reverse transcriptase polymerase chain reaction (RT-PCR), cloned and sequenced. The deduced amino acid sequence has 97.6% similarity with bovine Flt3-L isoform 1 and shares the long cytoplasmic domain observed in bovine Flt3-L but not in human Flt3-L.

U2 - 10.1111/j.1744-313X.2007.00659.x

DO - 10.1111/j.1744-313X.2007.00659.x

M3 - Article

SN - 1744-3121

VL - 34

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EP - 171

JO - International Journal of Immunogenetics

JF - International Journal of Immunogenetics

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