Cohesin-mediated genome architecture does not define DNA replication timing domains

Phoebe Oldach, Conrad A. Nieduszynski

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

3D genome organization is strongly predictive of DNA replication timing in mammalian cells. This work tested the extent to which loop-based genome architecture acts as a regulatory unit of replication timing by using an auxin-inducible system for acute cohesin ablation. Cohesin ablation in a population of cells in asynchronous culture was shown not to disrupt patterns of replication timing as assayed by replication sequencing (RepliSeq) or BrdU-focus microscopy. Furthermore, cohesin ablation prior to S phase entry in synchronized cells was similarly shown to not impact replication timing patterns. These results suggest that cohesin-mediated genome architecture is not required for the execution of replication timing patterns in S phase, nor for the establishment of replication timing domains in G1.

Original languageEnglish
Article number196
JournalGenes
Volume10
Issue number3
DOIs
Publication statusPublished - 4 Mar 2019

Keywords

  • Chromatin interactions
  • Cohesin
  • DNA
  • Genome architecture
  • Replication timing

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