TY - JOUR
T1 - Comparative genomics of Vibrio Cholerae from Haiti, Asia, and Africa
AU - Reimer, Aleisha R.
AU - van Domselaar, Gary
AU - Stroika, Steven
AU - Walker, Matthew
AU - Kent, Heather
AU - Tarr, Cheryl
AU - Talkington, Deborah
AU - Rowe, Lori
AU - Olsen-Rasmussen, Melissa
AU - Frace, Michael
AU - Sammons, Scott
AU - Dahourou, Georges Anicet
AU - Boncy, Jacques
AU - Smith, Anthony M.
AU - Mabon, Philip
AU - Petkau, Aaron
AU - Graham, Morag
AU - Gilmour, Matthew W.
AU - Gerner-Smidt, Peter
PY - 2011/11
Y1 - 2011/11
N2 - Cholera was absent from the island of Hispaniola at least a century before an outbreak that began in Haiti in the fall of 2010. Pulsed-field gel electrophoresis (PFGE) analysis of clinical isolates from the Haiti outbreak and recent global travelers returning to the United States showed indistinguishable PFGE fingerprints. To better explore the genetic ancestry of the Haiti outbreak strain, we acquired 23 whole-genome Vibrio cholerae sequences: 9 isolates obtained in Haiti or the Dominican Republic, 12 PFGE pattern-matched isolates linked to Asia or Africa, and 2 non matched outliers from the Western Hemisphere. Phylogenies for whole-genome sequences and core genome single-nucleotide polymorphisms showed that the Haiti outbreak strain is genetically related to strains originating in India and Cameroon. However, because no identical genetic match was found among sequenced contemporary isolates, a definitive genetic origin for the outbreak in Haiti remains speculative.
AB - Cholera was absent from the island of Hispaniola at least a century before an outbreak that began in Haiti in the fall of 2010. Pulsed-field gel electrophoresis (PFGE) analysis of clinical isolates from the Haiti outbreak and recent global travelers returning to the United States showed indistinguishable PFGE fingerprints. To better explore the genetic ancestry of the Haiti outbreak strain, we acquired 23 whole-genome Vibrio cholerae sequences: 9 isolates obtained in Haiti or the Dominican Republic, 12 PFGE pattern-matched isolates linked to Asia or Africa, and 2 non matched outliers from the Western Hemisphere. Phylogenies for whole-genome sequences and core genome single-nucleotide polymorphisms showed that the Haiti outbreak strain is genetically related to strains originating in India and Cameroon. However, because no identical genetic match was found among sequenced contemporary isolates, a definitive genetic origin for the outbreak in Haiti remains speculative.
UR - http://www.scopus.com/inward/record.url?scp=84930485738&partnerID=8YFLogxK
U2 - 10.3201/eid1711.110794
DO - 10.3201/eid1711.110794
M3 - Article
AN - SCOPUS:84930485738
SN - 1080-6040
VL - 17
SP - 2113
EP - 2121
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
IS - 11
ER -