Complete proton transfer cycle in GFP and its T203V and S205V mutants

Sergey P. Laptenok, Andras Lukacs, Agnieszka Gil, Richard Brust, Igor V. Sazanovich, Gregory M. Greetham, Peter J. Tonge, Stephen R. Meech

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Abstract

Proton transfer is critical in many important biochemical reactions. The unique three‐step excited‐state proton transfer in avGFP allows observations of protein proton transport in real‐time. In this work we exploit femtosecond to microsecond transient IR spectroscopy to record, in D2O, the complete proton transfer photocycle of avGFP, and two mutants (T203V and S205V) which modify the structure of the proton wire. Striking differences and similarities are observed among the three mutants yielding novel information on proton transfer mechanism, rates, isotope effects, H‐bond strength and proton wire stability. These data provide a detailed picture of the dynamics of long‐range proton transfer in a protein against which calculations may be compared.
Original languageEnglish
Pages (from-to)9303-9307
Number of pages5
JournalAngewandte Chemie International Edition
Volume54
Issue number32
Early online date18 Jun 2015
DOIs
Publication statusPublished - 3 Aug 2015

Keywords

  • Grün fluoreszierendes Protein (GFP)
  • IR-Spektroskopie
  • Kinetischer Isotopeneffekt
  • Protonentransfer
  • Ultraschnelle Spektroskopie

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