Conditions for efficient isolation and regeneration of protoplasts from Fulvia fulva

R. Harling; L. Kenyon; B. G. Lewis; R. P. Oliver; J. G. Turner; A. Coddington

doi:10.1111/j.1439-0434.1988.tb01001.x

Conditions for efficient isolation and regeneration of protoplasts from Fulvia fulva

R. Harling, L. Kenyon, B. G. Lewis, R. P. Oliver, J. G. Turner, A. Coddington

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

A procedure for producing high yields and high regeneration frequencies of protoplasts from Fulvia fulva is described. This procedure was devised by systematic trials of various parameters, such as culture age and osmotic support, which are known to affect protoplast yield and regeneration. Mycelium from liquid cultures of 24–48 h, incubated with Novozym 234 in buffered 1.0 M MgSO4, gave the best conditions for protoplast release. Regeneration frequencies up to 50% were obtained with a complete medium containing 0.8 M sucrose for osmotic support.

Original language	English
Pages (from-to)	143-146
Number of pages	4
Journal	Journal of Phytopathology
Volume	122
Issue number	2
DOIs	https://doi.org/10.1111/j.1439-0434.1988.tb01001.x
Publication status	Published - Jun 1988

Access to Document

10.1111/j.1439-0434.1988.tb01001.x

Cite this

@article{755ca296b4714f1ea5b73e9b47f4b4bb,

title = "Conditions for efficient isolation and regeneration of protoplasts from Fulvia fulva",

abstract = "A procedure for producing high yields and high regeneration frequencies of protoplasts from Fulvia fulva is described. This procedure was devised by systematic trials of various parameters, such as culture age and osmotic support, which are known to affect protoplast yield and regeneration. Mycelium from liquid cultures of 24–48 h, incubated with Novozym 234 in buffered 1.0 M MgSO4, gave the best conditions for protoplast release. Regeneration frequencies up to 50% were obtained with a complete medium containing 0.8 M sucrose for osmotic support.",

author = "R. Harling and L. Kenyon and Lewis, {B. G.} and Oliver, {R. P.} and Turner, {J. G.} and A. Coddington",

year = "1988",

month = jun,

doi = "10.1111/j.1439-0434.1988.tb01001.x",

language = "English",

volume = "122",

pages = "143--146",

journal = "Journal of Phytopathology",

issn = "0931-1785",

publisher = "Wiley",

number = "2",

}

TY - JOUR

T1 - Conditions for efficient isolation and regeneration of protoplasts from Fulvia fulva

AU - Harling, R.

AU - Kenyon, L.

AU - Lewis, B. G.

AU - Oliver, R. P.

AU - Turner, J. G.

AU - Coddington, A.

PY - 1988/6

Y1 - 1988/6

N2 - A procedure for producing high yields and high regeneration frequencies of protoplasts from Fulvia fulva is described. This procedure was devised by systematic trials of various parameters, such as culture age and osmotic support, which are known to affect protoplast yield and regeneration. Mycelium from liquid cultures of 24–48 h, incubated with Novozym 234 in buffered 1.0 M MgSO4, gave the best conditions for protoplast release. Regeneration frequencies up to 50% were obtained with a complete medium containing 0.8 M sucrose for osmotic support.

AB - A procedure for producing high yields and high regeneration frequencies of protoplasts from Fulvia fulva is described. This procedure was devised by systematic trials of various parameters, such as culture age and osmotic support, which are known to affect protoplast yield and regeneration. Mycelium from liquid cultures of 24–48 h, incubated with Novozym 234 in buffered 1.0 M MgSO4, gave the best conditions for protoplast release. Regeneration frequencies up to 50% were obtained with a complete medium containing 0.8 M sucrose for osmotic support.

U2 - 10.1111/j.1439-0434.1988.tb01001.x

DO - 10.1111/j.1439-0434.1988.tb01001.x

M3 - Article

SN - 0931-1785

VL - 122

SP - 143

EP - 146

JO - Journal of Phytopathology

JF - Journal of Phytopathology

IS - 2

ER -