Conjugated linoleic acid alters global gene expression in human intestinal-like Caco-2 cells in an isomer-specific manner

Eileen F Murphy, Guido J Hooiveld, Michael Muller, Raffaele A Calogero, Kevin D Cashman

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Conjugated linoleic acid (CLA) exhibits isomer-specific effects on transepithelial calcium (Ca) transport as well as on cell growth in human intestinal-like Caco-2 cells. However, the molecular mechanisms of action are still unclear. Therefore, this study used a transcriptomic approach to help elucidate the molecular mechanisms underlying such isomer-specific effects. Caco-2 cells were treated with 80 micromol/L linoleic acid (control), 80 micromol/L trans-10, cis-12 CLA, or 80 micromol/L cis-9, trans-11 CLA for 12 d. Ca transport was measured radio-isotopically. RNA was isolated from the cells, labeled, and hybridized to the Affymetrix U133 2.0 Plus arrays (n = 3). Data and functional analysis was preformed using Bioconductor. Using a minimum fold-change criterion of 1.6 and a false discovery rate criterion of P-value
Original languageEnglish
Pages (from-to)2359-2365
Number of pages7
JournalJournal of Nutrition
Volume137
Issue number11
DOIs
Publication statusPublished - Nov 2007

Keywords

  • Adenocarcinoma
  • Calcium
  • Tumor Cell Line
  • Colonic Neoplasms
  • Computational Biology
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Intestinal Mucosa
  • Conjugated
  • Oligonucleotide Array Sequence Analysis
  • Neoplasm RNA
  • Structure-Activity Relationship

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