Conjugated linoleic acid enhances transepithelial calcium transport in human intestinal-like Caco-2 cells: an insight into molecular changes

Eileen F Murphy, Christopher Jewell, Guido J Hooiveld, Michael Muller, Kevin D Cashman

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Conjugated linoleic acid (CLA) has been shown to enhance paracellular and transcellular Ca transport across human intestinal-like Caco-2 cell monolayers. The mechanisms of action, however, are still unclear. Therefore, this study investigated the molecular mechanisms underlying CLA-induced stimulation of Ca transport by use of preliminary microarray data together with more detailed and comprehensive quantitative reverse transcriptase-PCR analysis. While molecular expression of junctional adhesion molecule (JAM), ZO-2, ZO-3, claudin 2 and claudin 3 were unaltered, ZO-1, occludin, and claudin 4 were all up-regulated (1.6, 1.6, 2.4-fold, respectively; P
Original languageEnglish
Pages (from-to)295-301
Number of pages7
JournalProstaglandins, Leukotrienes and Essential Fatty Acids
Volume74
Issue number5
DOIs
Publication statusPublished - May 2006

Keywords

  • Biological Transport
  • Caco-2 Cells
  • Calcium
  • Calcium Channels
  • Cell Membrane Permeability
  • Claudin-1
  • Claudin-4
  • Gene Expression
  • Humans
  • Intestinal Mucosa
  • Linoleic Acids, Conjugated
  • Membrane Proteins
  • Occludin
  • Oligonucleotide Array Sequence Analysis
  • Tight Junctions

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