Conjugated linoleic acid enhances transepithelial calcium transport in human intestinal-like Caco-2 cells: an insight into molecular changes

Eileen F Murphy; Christopher Jewell; Guido J Hooiveld; Michael Muller; Kevin D Cashman

doi:10.1016/j.plefa.2006.03.003

Conjugated linoleic acid enhances transepithelial calcium transport in human intestinal-like Caco-2 cells: an insight into molecular changes

Eileen F Murphy, Christopher Jewell, Guido J Hooiveld, Michael Muller, Kevin D Cashman

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Conjugated linoleic acid (CLA) has been shown to enhance paracellular and transcellular Ca transport across human intestinal-like Caco-2 cell monolayers. The mechanisms of action, however, are still unclear. Therefore, this study investigated the molecular mechanisms underlying CLA-induced stimulation of Ca transport by use of preliminary microarray data together with more detailed and comprehensive quantitative reverse transcriptase-PCR analysis. While molecular expression of junctional adhesion molecule (JAM), ZO-2, ZO-3, claudin 2 and claudin 3 were unaltered, ZO-1, occludin, and claudin 4 were all up-regulated (1.6, 1.6, 2.4-fold, respectively; P

Original language	English
Pages (from-to)	295-301
Number of pages	7
Journal	Prostaglandins, Leukotrienes and Essential Fatty Acids
Volume	74
Issue number	5
DOIs	https://doi.org/10.1016/j.plefa.2006.03.003
Publication status	Published - May 2006

Keywords

Biological Transport
Caco-2 Cells
Calcium
Calcium Channels
Cell Membrane Permeability
Claudin-1
Claudin-4
Gene Expression
Humans
Intestinal Mucosa
Linoleic Acids, Conjugated
Membrane Proteins
Occludin
Oligonucleotide Array Sequence Analysis
Tight Junctions

Access to Document

10.1016/j.plefa.2006.03.003

Cite this

@article{5e7a6d3be7ea482baf8403a859dcb9fa,

title = "Conjugated linoleic acid enhances transepithelial calcium transport in human intestinal-like Caco-2 cells: an insight into molecular changes",

abstract = "Conjugated linoleic acid (CLA) has been shown to enhance paracellular and transcellular Ca transport across human intestinal-like Caco-2 cell monolayers. The mechanisms of action, however, are still unclear. Therefore, this study investigated the molecular mechanisms underlying CLA-induced stimulation of Ca transport by use of preliminary microarray data together with more detailed and comprehensive quantitative reverse transcriptase-PCR analysis. While molecular expression of junctional adhesion molecule (JAM), ZO-2, ZO-3, claudin 2 and claudin 3 were unaltered, ZO-1, occludin, and claudin 4 were all up-regulated (1.6, 1.6, 2.4-fold, respectively; P",

keywords = "Biological Transport, Caco-2 Cells, Calcium, Calcium Channels, Cell Membrane Permeability, Claudin-1, Claudin-4, Gene Expression, Humans, Intestinal Mucosa, Linoleic Acids, Conjugated, Membrane Proteins, Occludin, Oligonucleotide Array Sequence Analysis, Tight Junctions",

author = "Murphy, {Eileen F} and Christopher Jewell and Hooiveld, {Guido J} and Michael Muller and Cashman, {Kevin D}",

year = "2006",

month = may,

doi = "10.1016/j.plefa.2006.03.003",

language = "English",

volume = "74",

pages = "295--301",

journal = "Prostaglandins, Leukotrienes and Essential Fatty Acids",

issn = "0952-3278",

publisher = "Churchill Livingstone",

number = "5",

}

Conjugated linoleic acid enhances transepithelial calcium transport in human intestinal-like Caco-2 cells : an insight into molecular changes. / Murphy, Eileen F; Jewell, Christopher; Hooiveld, Guido J; Muller, Michael; Cashman, Kevin D.

In: Prostaglandins, Leukotrienes and Essential Fatty Acids, Vol. 74, No. 5, 05.2006, p. 295-301.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Conjugated linoleic acid enhances transepithelial calcium transport in human intestinal-like Caco-2 cells

T2 - an insight into molecular changes

AU - Murphy, Eileen F

AU - Jewell, Christopher

AU - Hooiveld, Guido J

AU - Muller, Michael

AU - Cashman, Kevin D

PY - 2006/5

Y1 - 2006/5

N2 - Conjugated linoleic acid (CLA) has been shown to enhance paracellular and transcellular Ca transport across human intestinal-like Caco-2 cell monolayers. The mechanisms of action, however, are still unclear. Therefore, this study investigated the molecular mechanisms underlying CLA-induced stimulation of Ca transport by use of preliminary microarray data together with more detailed and comprehensive quantitative reverse transcriptase-PCR analysis. While molecular expression of junctional adhesion molecule (JAM), ZO-2, ZO-3, claudin 2 and claudin 3 were unaltered, ZO-1, occludin, and claudin 4 were all up-regulated (1.6, 1.6, 2.4-fold, respectively; P

AB - Conjugated linoleic acid (CLA) has been shown to enhance paracellular and transcellular Ca transport across human intestinal-like Caco-2 cell monolayers. The mechanisms of action, however, are still unclear. Therefore, this study investigated the molecular mechanisms underlying CLA-induced stimulation of Ca transport by use of preliminary microarray data together with more detailed and comprehensive quantitative reverse transcriptase-PCR analysis. While molecular expression of junctional adhesion molecule (JAM), ZO-2, ZO-3, claudin 2 and claudin 3 were unaltered, ZO-1, occludin, and claudin 4 were all up-regulated (1.6, 1.6, 2.4-fold, respectively; P

KW - Biological Transport

KW - Caco-2 Cells

KW - Calcium

KW - Calcium Channels

KW - Cell Membrane Permeability

KW - Claudin-1

KW - Claudin-4

KW - Gene Expression

KW - Humans

KW - Intestinal Mucosa

KW - Linoleic Acids, Conjugated

KW - Membrane Proteins

KW - Occludin

KW - Oligonucleotide Array Sequence Analysis

KW - Tight Junctions

U2 - 10.1016/j.plefa.2006.03.003

DO - 10.1016/j.plefa.2006.03.003

M3 - Article

C2 - 16650747

VL - 74

SP - 295

EP - 301

JO - Prostaglandins, Leukotrienes and Essential Fatty Acids

JF - Prostaglandins, Leukotrienes and Essential Fatty Acids

SN - 0952-3278

IS - 5

ER -