Conserved SNH domain of the proto-oncoprotein SYT interacts with components of the human chromatin remodelling complexes, while the QPGY repeat domain forms homo-oligomers

Michela Perani, Catherine J E Ingram, Colin S Cooper, Michelle D Garrett, Graham H Goodwin

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Many studies have now established that the SWI/SNF chromatin remodelling complexes are involved in activation and repression of a variety of genes. In mammalian cells, these complexes contain the BRM and BRG1 helicase-like proteins that are thought to be responsible for nucleosome remodelling. The proto-oncoprotein SYT, involved in the unique translocation t(X;18) found in synovial sarcoma, is known to interact with human BRM (hBRM), thus providing a link between chromatin remodelling factors and human cancer. In this work, we address how SYT interacts with hBRM and BRG1. We demonstrate that the conserved N-terminal SNH domain of SYT, which is also present in the oncoproteins SYT-SSX, binds to both hBRM and BRG1. We have also found that in vivo the C-terminus transactivation QPGY region of SYT can interact with itself. This results in an amplified interaction with hBRM and highlights a possible regulatory function of this domain in cells.
Original languageEnglish
Pages (from-to)8156-67
Number of pages12
JournalOncogene
Volume22
Issue number50
DOIs
Publication statusPublished - 6 Nov 2003

Keywords

  • Amino Acid Sequence
  • Animals
  • COS Cells
  • Chromatin
  • Conserved Sequence
  • Humans
  • Neurotensin
  • Protein Structure, Tertiary
  • Proteins
  • Proto-Oncogene Proteins
  • Repressor Proteins
  • Transcription Factors
  • Vasoactive Intestinal Peptide

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