Control of bacterial nitrate assimilation by stabilization of G-quadruplex DNA

Zoë A. E. Waller; Benjamin J. Pinchbeck; Bhovina Seewoodharry Buguth; Timothy G. Meadows; David J. Richardson; Andrew J. Gates

doi:10.1039/c6cc06057a

Control of bacterial nitrate assimilation by stabilization of G-quadruplex DNA

Zoë A. E. Waller
, Benjamin J. Pinchbeck
, Bhovina Seewoodharry Buguth
, Timothy G. Meadows
, David J. Richardson
, Andrew J. Gates

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

52 Downloads (Pure)

Abstract

Here we present a chemical-biology study in the model soil bacterium Paracoccus denitrificans, where we show ligand-specific control of nitrate assimilation. Stabilization of a G-quadruplex in the promoter region of the nas genes, encoding the assimilatory nitrate/nitrite reductase system, is achieved using known quadruplex ligands and results in attenuation of gene transcription.

Original language	English
Pages (from-to)	13511-13514
Number of pages	4
Journal	Chemical Communications
Volume	52
Issue number	92
Early online date	1 Nov 2016
DOIs	https://doi.org/10.1039/c6cc06057a
Publication status	Published - 28 Nov 2016

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10.1039/c6cc06057aLicence: CC BY

Published manuscript
This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Final published version, 1.85 MBLicence: CC BY

Andrew Gates
- A.Gatesuea.acuk
- School of Biological Sciences - Associate Professor in Bacterial Bioenergetics
- Centre for Molecular and Structural Biochemistry - Member
- Molecular Microbiology - Member
- Wolfson Centre for Advanced Environmental Microbiology - Member
Person: Research Group Member, Research Centre Member, Academic, Teaching and Research

Investigating the stability and function of i-motif DNA.
Waller, Z. (Principal Investigator)
Biotechnology and Biological Sciences Research Council
30/11/14 → 29/12/17
Project: Research
Investigating widespread regulation of nitrogen assimilation at the level of RNA in bacteria
Gates, A. (Principal Investigator) & Lyall, V. (Technician)
Biotechnology and Biological Sciences Research Council
17/11/14 → 16/02/18
Project: Research

Cite this

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title = "Control of bacterial nitrate assimilation by stabilization of G-quadruplex DNA",

abstract = "Here we present a chemical-biology study in the model soil bacterium Paracoccus denitrificans, where we show ligand-specific control of nitrate assimilation. Stabilization of a G-quadruplex in the promoter region of the nas genes, encoding the assimilatory nitrate/nitrite reductase system, is achieved using known quadruplex ligands and results in attenuation of gene transcription.",

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AU - Pinchbeck, Benjamin J.

AU - Buguth, Bhovina Seewoodharry

AU - Meadows, Timothy G.

AU - Richardson, David J.

AU - Gates, Andrew J.

N1 - This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

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AB - Here we present a chemical-biology study in the model soil bacterium Paracoccus denitrificans, where we show ligand-specific control of nitrate assimilation. Stabilization of a G-quadruplex in the promoter region of the nas genes, encoding the assimilatory nitrate/nitrite reductase system, is achieved using known quadruplex ligands and results in attenuation of gene transcription.

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JO - Chemical Communications

JF - Chemical Communications

IS - 92

ER -

Control of bacterial nitrate assimilation by stabilization of G-quadruplex DNA

Abstract

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Profiles

Andrew Gates

Projects

Investigating the stability and function of i-motif DNA.

Investigating widespread regulation of nitrogen assimilation at the level of RNA in bacteria

Cite this