Cryo-EM structure of the four-subunit Rhodobacter sphaeroides cytochrome bc1 complex in styrene maleic acid nanodiscs

David J.K. Swainsbury, Frederick R. Hawkings, Elizabeth C. Martin, Sabina Musiał, Jack H. Salisbury, Philip J. Jackson, David A. Farmer, Matthew P. Johnson, C. Alistair Siebert, Andrew Hitchcock, C. Neil Hunter

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9 Citations (Scopus)

Abstract

Cytochrome bc1 complexes are ubiquinol:cytochrome c oxidoreductases, and as such, they are centrally important components of respiratory and photosynthetic electron transfer chains in many species of bacteria and in mitochondria. The minimal complex has three catalytic components, which are cytochrome b, cytochrome c1, and the Rieske iron–sulfur subunit, but the function of mitochondrial cytochrome bc1 complexes is modified by up to eight supernumerary subunits. The cytochrome bc1 complex from the purple phototrophic bacterium Rhodobacter sphaeroides has a single supernumerary subunit called subunit IV, which is absent from current structures of the complex. In this work we use the styrene–maleic acid copolymer to purify the R. sphaeroides cytochrome bc1 complex in native lipid nanodiscs, which retains the labile subunit IV, annular lipids, and natively bound quinones. The catalytic activity of the four-subunit cytochrome bc1 complex is threefold higher than that of the complex lacking subunit IV. To understand the role of subunit IV, we determined the structure of the four-subunit complex at 2.9 Å using single particle cryogenic electron microscopy. The structure shows the position of the transmembrane domain of subunit IV, which lies across the transmembrane helices of the Rieske and cytochrome c1 subunits. We observe a quinone at the Qo quinone-binding site and show that occupancy of this site is linked to conformational changes in the Rieske head domain during catalysis. Twelve lipids were structurally resolved, making contacts with the Rieske and cytochrome b subunits, with some spanning both of the two monomers that make up the dimeric complex.

Original languageEnglish
Article numbere2217922120
JournalProceedings of the National Academy of Sciences of the United States of America
Volume120
Issue number12
Early online date13 Mar 2023
DOIs
Publication statusPublished - 21 Mar 2023

Keywords

  • cytochrome bc
  • evolution
  • photosynthesis
  • quinone
  • Rhodobacter sphaeroides

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