The Brn-5 protein plays an important role in the control of cellular development and belongs to a class of transcription factors that usually contain two domains: the POU homeodomain (POU) and the POU-specific domain (POU). Since high-quality crystals suitable for crystallographic studies of the proteins of this class are difficult to obtain, all the known structural information available is for POU and/or POU. This paper describes several critical steps that allowed the production of high-quality crystals of the full-length Brn-5 protein complexed with its cognate DNA.
|Number of pages||4|
|Journal||Acta Crystallographica Section F Structural Biology and Crystallization Communications|
|Publication status||Published - 1 Jan 2008|
- transcription factors
- protein-DNA crystallization
- POU family
- Brn-5 protein