Crystallization and preliminary X-ray analysis of human Brn-5 transcription factor in complex with DNA

J.H. Pereira, S.C. Ha, S.-H. Kim

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3 Citations (Scopus)


The Brn-5 protein plays an important role in the control of cellular development and belongs to a class of transcription factors that usually contain two domains: the POU homeodomain (POU) and the POU-specific domain (POU). Since high-quality crystals suitable for crystallographic studies of the proteins of this class are difficult to obtain, all the known structural information available is for POU and/or POU. This paper describes several critical steps that allowed the production of high-quality crystals of the full-length Brn-5 protein complexed with its cognate DNA.
Original languageEnglish
Pages (from-to)175-178
Number of pages4
JournalActa Crystallographica Section F Structural Biology and Crystallization Communications
Issue number3
Publication statusPublished - 1 Jan 2008


  • transcription factors
  • protein-DNA crystallization
  • POU family
  • Brn-5 protein

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