Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa

Stuart A. Wilson; Naomi E. Chayen; Andrew M. Hemmings; Robert E. Drew; Laurence H. Pearl

doi:10.1016/0022-2836(91)90579-U

Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa

Stuart A. Wilson, Naomi E. Chayen, Andrew M. Hemmings, Robert E. Drew, Laurence H. Pearl

Research output: Contribution to journal › Article › peer-review

Abstract

The negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa has been purified from an over-expressing clone and crystalized. Crystals of diffraction quality were obtained from polyethylene glycol 4000 and ammonium sulphate. AmiC crystallizes in P4₂2₁2 (a = 104·4 A ̊, c = 66·6 A ̊) with one subunit in the asymmetric unit. Crystals diffract beyond 2·8 Å.

Original language	English
Pages (from-to)	869-871
Number of pages	3
Journal	Journal of Molecular Biology
Volume	222
Issue number	4
DOIs	https://doi.org/10.1016/0022-2836(91)90579-U
Publication status	Published - 20 Dec 1991
Externally published	Yes

Keywords

amidase
anti-termination mechanism
catabolic operon
negative regulator
P. aeruginosa

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10.1016/0022-2836(91)90579-U

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title = "Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa",

abstract = "The negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa has been purified from an over-expressing clone and crystalized. Crystals of diffraction quality were obtained from polyethylene glycol 4000 and ammonium sulphate. AmiC crystallizes in P42212 (a = 104·4 A ̊, c = 66·6 A ̊) with one subunit in the asymmetric unit. Crystals diffract beyond 2·8 {\AA}.",

keywords = "amidase, anti-termination mechanism, catabolic operon, negative regulator, P. aeruginosa",

author = "Wilson, {Stuart A.} and Chayen, {Naomi E.} and Hemmings, {Andrew M.} and Drew, {Robert E.} and Pearl, {Laurence H.}",

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doi = "10.1016/0022-2836(91)90579-U",

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T1 - Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa

AU - Wilson, Stuart A.

AU - Chayen, Naomi E.

AU - Hemmings, Andrew M.

AU - Drew, Robert E.

AU - Pearl, Laurence H.

PY - 1991/12/20

Y1 - 1991/12/20

N2 - The negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa has been purified from an over-expressing clone and crystalized. Crystals of diffraction quality were obtained from polyethylene glycol 4000 and ammonium sulphate. AmiC crystallizes in P42212 (a = 104·4 A ̊, c = 66·6 A ̊) with one subunit in the asymmetric unit. Crystals diffract beyond 2·8 Å.

AB - The negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa has been purified from an over-expressing clone and crystalized. Crystals of diffraction quality were obtained from polyethylene glycol 4000 and ammonium sulphate. AmiC crystallizes in P42212 (a = 104·4 A ̊, c = 66·6 A ̊) with one subunit in the asymmetric unit. Crystals diffract beyond 2·8 Å.

KW - amidase

KW - anti-termination mechanism

KW - catabolic operon

KW - negative regulator

KW - P. aeruginosa

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U2 - 10.1016/0022-2836(91)90579-U

DO - 10.1016/0022-2836(91)90579-U

M3 - Article

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AN - SCOPUS:0026335459

SN - 0022-2836

VL - 222

SP - 869

EP - 871

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 4

ER -

Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa

Abstract

Keywords

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