TY - JOUR
T1 - Crystallization of the cytotoxic domain of a ribosome-inactivating colicin in complex with its immunity protein
AU - Carr, S.
AU - Walker, D.
AU - James, R.
AU - Kleanthous, C.
AU - Hemmings, A. M.
PY - 2000
Y1 - 2000
N2 - The complex between the ribonuclease domain of the ribosome-inactivating colicin E3 and its protein inhibitor, the cognate immunity Im3, has been crystallized and preliminary X-ray characterization has been performed. Single crystals of the 1:1 complex were grown from hanging-drop vapour-diffusion experiments using 2-propanol as a precipitant. The space group is P3121 or P3221, with unit-cell parameters a = b = 93.7, c = 76.2 Å. When cryocooled, these crystals diffract to a resolution of 2.4 Å. A search for suitable conventional heavy-atom derivatives was unsuccessful and so Im3 mutants containing engineered cysteine or methionine residues have been produced for mercury soaks and selenomethionine-labelling experiments, respectively.
AB - The complex between the ribonuclease domain of the ribosome-inactivating colicin E3 and its protein inhibitor, the cognate immunity Im3, has been crystallized and preliminary X-ray characterization has been performed. Single crystals of the 1:1 complex were grown from hanging-drop vapour-diffusion experiments using 2-propanol as a precipitant. The space group is P3121 or P3221, with unit-cell parameters a = b = 93.7, c = 76.2 Å. When cryocooled, these crystals diffract to a resolution of 2.4 Å. A search for suitable conventional heavy-atom derivatives was unsuccessful and so Im3 mutants containing engineered cysteine or methionine residues have been produced for mercury soaks and selenomethionine-labelling experiments, respectively.
UR - http://www.scopus.com/inward/record.url?scp=0034520580&partnerID=8YFLogxK
U2 - 10.1107/S0907444900010726
DO - 10.1107/S0907444900010726
M3 - Article
C2 - 11092930
AN - SCOPUS:0034520580
SN - 0907-4449
VL - 56
SP - 1630
EP - 1633
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 12
ER -