Cytokine release by lipopolysaccharide-stimulated whole blood from patients with typhoid fever

Deborah House, Nguyen T. Chinh, Tran T. Hien, Christopher P. Parry, Nguyen T. Ly, To S. Diep, John Wain, Sarah Dunstan, Nicholas J. White, Gordon Dougan, Jeremy J. Farrar

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18 Citations (Scopus)

Abstract

The ex vivo cytokine response to lipopolysaccharide (LPS) of whole blood from patients with typhoid fever was investigated. Tumor necrosis factor (TNF)–a release by LPS-stimulated blood was found to be lower during acute typhoid fever than after a course of antimicrobial therapy (P?.001). Ex vivo interleukin (IL)–1ß, but not IL-1 receptor antagonist, release was also depressed during the acute stage of typhoid fever. Low ex vivo production of TNF-a was associated with delayed recovery. No association was found between the TNFA-308 promoter polymorphism and LPS-induced TNF-a release, either during an active infection or after treatment. In acute typhoid fever, the ability of peripheral blood leukocytes to release proinflammatory cytokines in response to an inflammatory stimulus is depressed, and this may contribute to delayed recovery following antibiotic treatment
Original languageEnglish
Pages (from-to)240-245
Number of pages6
JournalJournal of Infectious Diseases
Volume186
Issue number2
DOIs
Publication statusPublished - 15 Jul 2002

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