Defining minimum genomic regions of imbalance involved in testicular germ cell tumors of adolescents and adults through genome wide microarray analysis of cDNA clones

Alan McIntyre; Brenda Summersgill; Osman Jafer; Sandrine Rodriguez; Gaetano Zafarana; J Wolter Oosterhuis; Ad Jm Gillis; Leendert Looijenga; Colin Cooper; Robert Huddart; Jeremy Clark; Janet Shipley

doi:10.1038/sj.onc.1208115

Defining minimum genomic regions of imbalance involved in testicular germ cell tumors of adolescents and adults through genome wide microarray analysis of cDNA clones

Alan McIntyre, Brenda Summersgill, Osman Jafer, Sandrine Rodriguez, Gaetano Zafarana, J Wolter Oosterhuis, Ad Jm Gillis, Leendert Looijenga, Colin Cooper, Robert Huddart, Jeremy Clark, Janet Shipley

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Identifying changes in DNA copy number can pinpoint genes that may be involved in tumor development. Here we have defined the smallest overlapping regions of imbalance (SORI) in testicular germ cell tumors other than the 12p region, which has been previously investigated. Definition of the regions was achieved through comparative genomic hybridization (CGH) analysis of a 4559 cDNA clone microarray. A total of 14 SORI were identified, which involved at least five of the 11 samples analysed. Many of these refined regions were previously reported using chromosomal or allelic imbalance studies. The SORI included gain of material from the regions 4q12, 17q21.3, 22q11.23 and Xq22, and loss from 5q33, 11q12.1, 16q22.3 and 22q11. Comparison with parallel chromosomal CGH data supported involvement of most regions. The various SORI span between one and 20 genes and highlight potential oncogenes/tumor suppressor genes to be investigated further.

Original language	English
Pages (from-to)	9142-7
Number of pages	6
Journal	Oncogene
Volume	23
Issue number	56
DOIs	https://doi.org/10.1038/sj.onc.1208115
Publication status	Published - 2 Dec 2004

Keywords

Adolescent
Adult
Chromosome Mapping
DNA, Complementary
Germinoma
Humans
Male
Oligonucleotide Array Sequence Analysis
Testicular Neoplasms

Access to Document

10.1038/sj.onc.1208115

Cite this

McIntyre, A., Summersgill, B., Jafer, O., Rodriguez, S., Zafarana, G., Oosterhuis, J. W., Gillis, A. J., Looijenga, L., Cooper, C., Huddart, R., Clark, J., & Shipley, J. (2004). Defining minimum genomic regions of imbalance involved in testicular germ cell tumors of adolescents and adults through genome wide microarray analysis of cDNA clones. Oncogene, 23(56), 9142-7. https://doi.org/10.1038/sj.onc.1208115

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title = "Defining minimum genomic regions of imbalance involved in testicular germ cell tumors of adolescents and adults through genome wide microarray analysis of cDNA clones",

abstract = "Identifying changes in DNA copy number can pinpoint genes that may be involved in tumor development. Here we have defined the smallest overlapping regions of imbalance (SORI) in testicular germ cell tumors other than the 12p region, which has been previously investigated. Definition of the regions was achieved through comparative genomic hybridization (CGH) analysis of a 4559 cDNA clone microarray. A total of 14 SORI were identified, which involved at least five of the 11 samples analysed. Many of these refined regions were previously reported using chromosomal or allelic imbalance studies. The SORI included gain of material from the regions 4q12, 17q21.3, 22q11.23 and Xq22, and loss from 5q33, 11q12.1, 16q22.3 and 22q11. Comparison with parallel chromosomal CGH data supported involvement of most regions. The various SORI span between one and 20 genes and highlight potential oncogenes/tumor suppressor genes to be investigated further.",

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author = "Alan McIntyre and Brenda Summersgill and Osman Jafer and Sandrine Rodriguez and Gaetano Zafarana and Oosterhuis, {J Wolter} and Gillis, {Ad Jm} and Leendert Looijenga and Colin Cooper and Robert Huddart and Jeremy Clark and Janet Shipley",

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McIntyre, A, Summersgill, B, Jafer, O, Rodriguez, S, Zafarana, G, Oosterhuis, JW, Gillis, AJ, Looijenga, L, Cooper, C, Huddart, R, Clark, J & Shipley, J 2004, 'Defining minimum genomic regions of imbalance involved in testicular germ cell tumors of adolescents and adults through genome wide microarray analysis of cDNA clones', Oncogene, vol. 23, no. 56, pp. 9142-7. https://doi.org/10.1038/sj.onc.1208115

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T1 - Defining minimum genomic regions of imbalance involved in testicular germ cell tumors of adolescents and adults through genome wide microarray analysis of cDNA clones

AU - McIntyre, Alan

AU - Summersgill, Brenda

AU - Jafer, Osman

AU - Rodriguez, Sandrine

AU - Zafarana, Gaetano

AU - Oosterhuis, J Wolter

AU - Gillis, Ad Jm

AU - Looijenga, Leendert

AU - Cooper, Colin

AU - Huddart, Robert

AU - Clark, Jeremy

AU - Shipley, Janet

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AB - Identifying changes in DNA copy number can pinpoint genes that may be involved in tumor development. Here we have defined the smallest overlapping regions of imbalance (SORI) in testicular germ cell tumors other than the 12p region, which has been previously investigated. Definition of the regions was achieved through comparative genomic hybridization (CGH) analysis of a 4559 cDNA clone microarray. A total of 14 SORI were identified, which involved at least five of the 11 samples analysed. Many of these refined regions were previously reported using chromosomal or allelic imbalance studies. The SORI included gain of material from the regions 4q12, 17q21.3, 22q11.23 and Xq22, and loss from 5q33, 11q12.1, 16q22.3 and 22q11. Comparison with parallel chromosomal CGH data supported involvement of most regions. The various SORI span between one and 20 genes and highlight potential oncogenes/tumor suppressor genes to be investigated further.

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KW - Adult

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KW - Germinoma

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KW - Male

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