The application is directed to the impedimetric determination of analyte binding, eg. DNA hybridisation using electrochemical impedance spectrometry (EIS). In one embodiment, simultaneous multi-frequency excitation is used, followed by FFT. In another, a differential method is used to monitor the rate of change of EIS variables, especially the electron transfer resistance, Ret. Combining EIS with labels allows the multiplex determination of a plurality of analytes. Also, kinetic monitoring of enzyme activity, especially of protease in wound healing, becomes possible.
|Publication status||Published - 1 Jun 2011|