Detection of Oxazolidinone-Resistant Enterococcus faecalis and Enterococcus faecium Strains by Real-Time PCR and PCR-Restriction Fragment Length Polymorphism Analysis

Neil Woodford, Luke Tysall, Cressida Auckland, Mark W. Stockdale, Andrew J. Lawson, Rachel A. Walker, David M. Livermore

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44 Citations (Scopus)

Abstract

A real-time PCR assay identified linezolid-resistant Enterococcus faecalis and Enterococcus faecium isolates with a G2576U rRNA mutation. PCR-restriction fragment length polymorphism analysis of ribosomal DNA amplicons with NheI also detected this mutation. Both assays detected isolates heterozygous at this position. Recognition of isolates with what is presently the most frequent oxazolidinone resistance mutation may aid surveillance and individual case management.
Original languageEnglish
Pages (from-to)4298-4300
Number of pages3
JournalJournal of Clinical Microbiology
Volume40
Issue number11
DOIs
Publication statusPublished - Nov 2002

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