Detection of TT virus by single-primer sequence-independent amplification in multiple samples collected from an outbreak of gastroenteritis

S Braham; M Iturriza-Gómara; J Gray

doi:10.1007/s00705-009-0387-8

Detection of TT virus by single-primer sequence-independent amplification in multiple samples collected from an outbreak of gastroenteritis

S Braham, M Iturriza-Gómara, J Gray

Norwich Medical School

Research output: Contribution to journal › Article

6 Citations (Scopus)

Abstract

A panel of gastroenteritis outbreak samples was subjected to a virus purification and concentration algorithm followed by a sequence-independent amplification method devised to detect viral enteric pathogens. The application of these methods allowed the identification of torque teno virus (TTV) in one outbreak. The complete genome sequence of 3,260 nt was obtained through "genome walking", and four open reading frames were deduced from the genomic sequence. Phylogenetic analysis grouped this virus in TTV genetic group 3, clustering with genotype 27, with 85% similarity at the nt level with strain SAa-01.

Original language	English
Pages (from-to)	981-985
Number of pages	5
Journal	Archives of Virology
Volume	154
Issue number	6
DOIs	https://doi.org/10.1007/s00705-009-0387-8
Publication status	Published - Jun 2009

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10.1007/s00705-009-0387-8

Cite this

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abstract = "A panel of gastroenteritis outbreak samples was subjected to a virus purification and concentration algorithm followed by a sequence-independent amplification method devised to detect viral enteric pathogens. The application of these methods allowed the identification of torque teno virus (TTV) in one outbreak. The complete genome sequence of 3,260 nt was obtained through {"}genome walking{"}, and four open reading frames were deduced from the genomic sequence. Phylogenetic analysis grouped this virus in TTV genetic group 3, clustering with genotype 27, with 85% similarity at the nt level with strain SAa-01.",

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AU - Iturriza-Gómara, M

AU - Gray, J

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N2 - A panel of gastroenteritis outbreak samples was subjected to a virus purification and concentration algorithm followed by a sequence-independent amplification method devised to detect viral enteric pathogens. The application of these methods allowed the identification of torque teno virus (TTV) in one outbreak. The complete genome sequence of 3,260 nt was obtained through "genome walking", and four open reading frames were deduced from the genomic sequence. Phylogenetic analysis grouped this virus in TTV genetic group 3, clustering with genotype 27, with 85% similarity at the nt level with strain SAa-01.

AB - A panel of gastroenteritis outbreak samples was subjected to a virus purification and concentration algorithm followed by a sequence-independent amplification method devised to detect viral enteric pathogens. The application of these methods allowed the identification of torque teno virus (TTV) in one outbreak. The complete genome sequence of 3,260 nt was obtained through "genome walking", and four open reading frames were deduced from the genomic sequence. Phylogenetic analysis grouped this virus in TTV genetic group 3, clustering with genotype 27, with 85% similarity at the nt level with strain SAa-01.

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DO - 10.1007/s00705-009-0387-8

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VL - 154

SP - 981

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JO - Archives of Virology

JF - Archives of Virology

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