TY - JOUR
T1 - Detection of TT virus by single-primer sequence-independent amplification in multiple samples collected from an outbreak of gastroenteritis
AU - Braham, S
AU - Iturriza-Gómara, M
AU - Gray, J
PY - 2009/6
Y1 - 2009/6
N2 - A panel of gastroenteritis outbreak samples was subjected to a virus purification and concentration algorithm followed by a sequence-independent amplification method devised to detect viral enteric pathogens. The application of these methods allowed the identification of torque teno virus (TTV) in one outbreak. The complete genome sequence of 3,260 nt was obtained through "genome walking", and four open reading frames were deduced from the genomic sequence. Phylogenetic analysis grouped this virus in TTV genetic group 3, clustering with genotype 27, with 85% similarity at the nt level with strain SAa-01.
AB - A panel of gastroenteritis outbreak samples was subjected to a virus purification and concentration algorithm followed by a sequence-independent amplification method devised to detect viral enteric pathogens. The application of these methods allowed the identification of torque teno virus (TTV) in one outbreak. The complete genome sequence of 3,260 nt was obtained through "genome walking", and four open reading frames were deduced from the genomic sequence. Phylogenetic analysis grouped this virus in TTV genetic group 3, clustering with genotype 27, with 85% similarity at the nt level with strain SAa-01.
U2 - 10.1007/s00705-009-0387-8
DO - 10.1007/s00705-009-0387-8
M3 - Article
VL - 154
SP - 981
EP - 985
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 6
ER -