Determination and characterization of Chenopodium quinoa willd. Saponins

S.G. Verza, S. Kaiser, P.E. de Resende, G.G. Ortega

Research output: Contribution to journalArticlepeer-review

Abstract

Chenopodium quinoa seeds are well-known for their high saponin content. From a crude hydroethanolic extract (EXQ) two enriched saponin fractions (FQ70 and FQ90) were prepared, their saponin content assayed and characterized by LC and MALDI-TOF/TOF, respectively. The LC-method consisted of a Gemini-NX RP C18 column and a mobile phase prepared with 0.1 % formic acid solution and acetonitrile in gradient elution. The flow rate was 1.2 mL/min and the detection at 210 nm. The method validation was throughout satisfactory. The analytical method was applied to quinoa extract and quinoa saponins fractions and was suitable to provide quantitative information for the quality control of them. The prevalence of phytolaccagenic acid derivatives was assessed by MALDI-TOF/TOF and saponins derived from hederagenin and serjanic acid were characterized too in Chenopodium quinoa saponins fractions.
Original languageEnglish
Pages (from-to)326-331
Number of pages6
JournalLatin American Journal of Pharmacy
Volume36
Issue number2
Publication statusPublished - 2017

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