Chenopodium quinoa seeds are well-known for their high saponin content. From a crude hydroethanolic extract (EXQ) two enriched saponin fractions (FQ70 and FQ90) were prepared, their saponin content assayed and characterized by LC and MALDI-TOF/TOF, respectively. The LC-method consisted of a Gemini-NX RP C18 column and a mobile phase prepared with 0.1 % formic acid solution and acetonitrile in gradient elution. The flow rate was 1.2 mL/min and the detection at 210 nm. The method validation was throughout satisfactory. The analytical method was applied to quinoa extract and quinoa saponins fractions and was suitable to provide quantitative information for the quality control of them. The prevalence of phytolaccagenic acid derivatives was assessed by MALDI-TOF/TOF and saponins derived from hederagenin and serjanic acid were characterized too in Chenopodium quinoa saponins fractions.
|Number of pages||6|
|Journal||Latin American Journal of Pharmacy|
|Publication status||Published - 2017|