Whole-mount in situ hybridization (WISH) is a technique that enables temporal and spatial visualization of RNA molecules in an embryo or whole tissue by using a complementary labelled probe. MicroRNAs are short noncoding RNAs of 20–25 nt in length mainly involved in posttranscriptional regulation of gene expression. In this chapter, we describe how to visualize miRNAs in Xenopus laevis or tropicalis by WISH using two different approaches: LNA-WISH to visualize mature miRNAs and pri-miRNA-WISH to visualize the immature form of miRNAs, the pri-miRNAs.
|Title of host publication||MicroRNA Detection and Target Identification|
|Subtitle of host publication||Methods and Protocols|
|Number of pages||10|
|Publication status||Published - 24 Jan 2023|
|Name||Methods in Molecular Biology|
- Expression pattern