@inbook{eb14c56af8cb45f189b8bd3fe7a0824a,
title = "Determining miRNA Expression Patterns in Xenopus",
abstract = "Whole-mount in situ hybridization (WISH) is a technique that enables temporal and spatial visualization of RNA molecules in an embryo or whole tissue by using a complementary labelled probe. MicroRNAs are short noncoding RNAs of 20–25 nt in length mainly involved in posttranscriptional regulation of gene expression. In this chapter, we describe how to visualize miRNAs in Xenopus laevis or tropicalis by WISH using two different approaches: LNA-WISH to visualize mature miRNAs and pri-miRNA-WISH to visualize the immature form of miRNAs, the pri-miRNAs.",
keywords = "Development, Expression pattern, WISH, Xenopus, miRNA",
author = "Marco Antonaci and Godden, {Alice M.} and Wheeler, {Grant N.}",
note = "Funding: MA is funded by the European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sk{\l}odowska-Curie grant agreement no. 860635. AMG was supported by UKRI Biotechnology and Biological Sciences Research Council Norwich Research Park Biosciences Doctoral Training Partnership grants (BB/M011216/1).",
year = "2023",
month = jan,
day = "24",
doi = "10.1007/978-1-0716-2982-6_11",
language = "English",
isbn = "978-1-0716-2981-9",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "145--154",
editor = "Tamas Dalmay",
booktitle = "MicroRNA Detection and Target Identification",
address = "United States",
}