Determining miRNA Expression Patterns in Xenopus

Research output: Chapter in Book/Report/Conference proceedingChapter


Whole-mount in situ hybridization (WISH) is a technique that enables temporal and spatial visualization of RNA molecules in an embryo or whole tissue by using a complementary labelled probe. MicroRNAs are short noncoding RNAs of 20–25 nt in length mainly involved in posttranscriptional regulation of gene expression. In this chapter, we describe how to visualize miRNAs in Xenopus laevis or tropicalis by WISH using two different approaches: LNA-WISH to visualize mature miRNAs and pri-miRNA-WISH to visualize the immature form of miRNAs, the pri-miRNAs.
Original languageEnglish
Title of host publicationMicroRNA Detection and Target Identification
Subtitle of host publicationMethods and Protocols
EditorsTamas Dalmay
PublisherHumana Press
Number of pages10
ISBN (Print)978-1-0716-2981-9
Publication statusPublished - 24 Jan 2023

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745


  • Development
  • Expression pattern
  • WISH
  • Xenopus
  • miRNA

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