@inbook{eb14c56af8cb45f189b8bd3fe7a0824a,
title = "Determining miRNA Expression Patterns in Xenopus",
abstract = "Whole-mount in situ hybridization (WISH) is a technique that enables temporal and spatial visualization of RNA molecules in an embryo or whole tissue by using a complementary labelled probe. MicroRNAs are short noncoding RNAs of 20{\textendash}25 nt in length mainly involved in posttranscriptional regulation of gene expression. In this chapter, we describe how to visualize miRNAs in Xenopus laevis or tropicalis by WISH using two different approaches: LNA-WISH to visualize mature miRNAs and pri-miRNA-WISH to visualize the immature form of miRNAs, the pri-miRNAs.",
keywords = "Development, Expression pattern, WISH, Xenopus, miRNA",
author = "Marco Antonaci and Godden, \{Alice M.\} and Wheeler, \{Grant N.\}",
note = "Funding: MA is funded by the European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sk{\l}odowska-Curie grant agreement no. 860635. AMG was supported by UKRI Biotechnology and Biological Sciences Research Council Norwich Research Park Biosciences Doctoral Training Partnership grants (BB/M011216/1).",
year = "2023",
month = jan,
day = "24",
doi = "10.1007/978-1-0716-2982-6\_11",
language = "English",
isbn = "978-1-0716-2981-9",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "145--154",
editor = "Tamas Dalmay",
booktitle = "MicroRNA Detection and Target Identification",
address = "United States",
}