Determining Shiga toxin-producing Escherichia coli interactions with human intestinal epithelium in a microaerobic vertical diffusion chamber

Conor J. McGrath; Stephanie Schuller

doi:10.1007/978-1-0716-1339-9_12

Determining Shiga toxin-producing Escherichia coli interactions with human intestinal epithelium in a microaerobic vertical diffusion chamber

Conor J. McGrath, Stephanie Schuller

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

2 Citations (Scopus)

Abstract

The environment in the human intestine is low in oxygen. This affects virulence gene expression of enteropathogens including Shiga toxin-producing E. coli (STEC) and enables mucosal colonization by oxygen-sensitive commensal bacteria. To simulate the oxygen-restricted milieu at the intestinal epithelium, we have developed a vertical diffusion chamber model (VDC) which allows infection of polarized human intestinal epithelia under microaerobic conditions. In this chapter, we present a detailed protocol for performing STEC infections in the VDC system and subsequent analysis of STEC pathogenesis.

Original language	English
Title of host publication	Shiga Toxin-Producing E. coli: Methods and Protocols
Publisher	Springer
Chapter	12
Pages	273-283
Number of pages	11
ISBN (Electronic)	978-1-0716-1339-9
ISBN (Print)	978-1-0716-1338-2
DOIs	https://doi.org/10.1007/978-1-0716-1339-9_12
Publication status	Published - Mar 2021

Access to Document

10.1007/978-1-0716-1339-9_12

2 Citations (Scopus)
1 Book

Shiga Toxin-Producing E. coli: Methods and Protocols
Schuller, S. & Bielaszewska, M., Mar 2021, Springer. 421 p.
Research output: Book/Report › Book

Cite this

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abstract = "The environment in the human intestine is low in oxygen. This affects virulence gene expression of enteropathogens including Shiga toxin-producing E. coli (STEC) and enables mucosal colonization by oxygen-sensitive commensal bacteria. To simulate the oxygen-restricted milieu at the intestinal epithelium, we have developed a vertical diffusion chamber model (VDC) which allows infection of polarized human intestinal epithelia under microaerobic conditions. In this chapter, we present a detailed protocol for performing STEC infections in the VDC system and subsequent analysis of STEC pathogenesis.",

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Determining Shiga toxin-producing Escherichia coli interactions with human intestinal epithelium in a microaerobic vertical diffusion chamber. / McGrath, Conor J.; Schuller, Stephanie.

Shiga Toxin-Producing E. coli: Methods and Protocols. Springer, 2021. p. 273-283.

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

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