Determining Shiga toxin-producing Escherichia coli interactions with human intestinal epithelium in a microaerobic vertical diffusion chamber

Conor J. McGrath, Stephanie Schuller

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

The environment in the human intestine is low in oxygen. This affects virulence gene expression of enteropathogens including Shiga toxin-producing E. coli (STEC) and enables mucosal colonization by oxygen-sensitive commensal bacteria. To simulate the oxygen-restricted milieu at the intestinal epithelium, we have developed a vertical diffusion chamber model (VDC) which allows infection of polarized human intestinal epithelia under microaerobic conditions. In this chapter, we present a detailed protocol for performing STEC infections in the VDC system and subsequent analysis of STEC pathogenesis.

Original languageEnglish
Title of host publicationShiga Toxin-Producing E. coli: Methods and Protocols
PublisherSpringer
Chapter12
Pages273-283
Number of pages11
ISBN (Electronic)978-1-0716-1339-9
ISBN (Print)978-1-0716-1338-2
DOIs
Publication statusPublished - Mar 2021

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